A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Ranbp11SH0971 Insertion

General Information
Symbol Dmel\P{lacW}Ranbp11SH0971 Species D. melanogaster
Name FlyBase ID FBti0026028
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) l(2)SH0971, Ranbp11 Viability / fertility
Causes allele(s) l(2)SH0971SH0971, Ranbp11SH0971 Stock availability 1 publicly available
LINE ID SH0971
Genomic Location
Chromosomal location 2R ( 52B5 ) Sequence location 2R:11,570,389..11,570,389 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0067807 FBti0068114 FBti0066551 FBti0046404 FBti0051488 FBti0067932 FBti0051739 FBti0046153 FBti0066709 FBti0115645 FBti0056210 FBti0109838 FBti0069509 FBti0054488 FBti0056661 FBti0069987 FBti0070191 FBti0054489 FBti0044470 FBti0035231 FBti0110517 FBti0109171 FBti0110356 FBti0040975 FBti0057823 FBti0065657 FBti0025947 FBti0111358 FBti0042251 FBti0115605 FBti0125197_2 FBti0125197_1 FBti0048690 FBti0106071 FBti0026028 FBti0037746 FBti0129180 FBti0100482
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:11,570,389..11,570,389 [-]
Orientation
Cytological location
(computed by FlyBase)
52B5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
52B5_r5 (reported as inferred from sequence location)
52C1-52C2 (reported as inferred from sequence location)
Comments concerning
location
Genomic location previously reported as 2R:11570382..11570438.
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
Size 10.691Kb
Associated alleles
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
lethal | recessive
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
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hide Comments
Location 2R:11197739-11197797 confirmed by FlyBase alignment of dbGSS accession AB082335 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{lacW}l(2)SH0971SH0971
P{lacW}Ranbp11SH0971
 
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Oh et al., 2003, Genetics 163(1): 195--201
A P-element insertion screen identified mutations in 455 novel essential genes in Drosophila. [FBrf0155812]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material
Oh et al., 2003, Genetics 163(1):
Table S1. [FBrf0157097]
Personal communication to FlyBase
Levis and Carlson, 2011.9.12, Updated location data for SH lines
Updated location data for SH lines [FBrf0215563]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]