Dmel\P{lacW}SNF4Aγ^loe Insertion

General Information
Symbol	Dmel\P{lacW}SNF4Aγ^loe	Species	D. melanogaster
Name		FlyBase ID	FBti0026458
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	SNF4Aγ	Viability / fertility
Causes allele(s)	SNF4Aγ^loe	Stock availability	none publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 93C1-93C5 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms eye
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	93C1-93C5 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	SNF4Aγ (Tschaepe et al., 2002, Wentzell et al., 2012, Cook et al., 2012)
Alleles and Phenotypes
Causes alleles	SNF4Aγ^loe (Tschaepe et al., 2002, Wentzell et al., 2012, Cook et al., 2012)

Lethality References
Sterility References
Phenotype Manifest In
	adult brain (Tschaepe et al., 2002, Wentzell et al., 2012) adult central complex (Tschaepe et al., 2002) adult central nervous system (Tschaepe et al., 2002) embryonic/larval brain \| third instar larval stage (Wentzell et al., 2012) eye (Cook et al., 2012)
Detailed Description
Statement Reference SNF4Aγ[loe] homo- and heterozygous mutants exhibit age-dependent degeneration of the nervous system. SNF4Aγ[loe] exhibit a mild rough eye phenotype. (Cook et al., 2012) A Fpps[k06103] heterozygous background significantly suppresses the degenerative nervous sytem phenotype found in 5 day old SNF4Aγ[loe] mutants. These flies exhibit a reduction in the area of vacuoles in the optic system, from approximately 163 υm[2] to 33υm[2] in a Fpps[k03514]/+ background and 25υm[2] in a Fpps[k06103]/+ background. There is a similar reduction in the number of vacuoles present, confirming an involvement of the isoprenoid pathway in the degenerative phenotype of SNF4Aγ[loe] flies. There is no significant difference in the nervous system degeneration seen in SNF4Aγ[loe] mutants in a Cdc42[3] or Rab5[k08230] heterozygous background. A heterozygous Rho1[72F] background suppresses the neuronal vacuolization seen in SNF4Aγ[loe] mutants to approximately half the level in single mutants. The number of vacuoles is also reduced, compared to SNF4Aγ[loe] single mutants. Rho1[72F] heterozygosity increases the performance index of SNF4Aγ[loe] flies in a fast phototaxis assay. Expression of constitutively-active Rho1[V14.Scer\UAS] pan-neuronally, under the control of Scer\GAL4[Appl.G1a], enhances the neural degeneration and vacuolization seen in SNF4Aγ[loe] mutants. Expression of Rho1[V14.Scer\UAS] under the control of Scer\GAL4[Appl.G1a] decreases the performance index of SNF4Aγ[loe] flies in a fast phototaxis assay. A SNF4Aγ[loe] mutant background does not affect the external or internal eye phenotype caused by overexpression of Rho1[V14.Scer\UAS] under the control of Scer\GAL4[GMR.PF]. Rho1[72F]/+; SNF4Aγ[loe]/SNF4Aγ[loe] flies show a significant increase in lifespan compared to SNF4Aγ[loe] mutants. (Cook et al., 2012) Expression of Hsap\APP[695.Scer\UAS.Exel] under the control of Scer\GAL4[Appl.G1a] dramatically suppresses the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Hsap\APP[s.α.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Appl.G1a] fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Hsap\APP[s.β.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Appl.G1a] fails to suppress the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Co-expression of Hsap\BACE1[Scer\UAS.cGa] suppresses the increase in vacuolisation seen when Hsap\APP[695.Scer\UAS.Exel] is expressed in the brains of SNF4Aγ[loe] mutant third instar larvae under the control of Scer\GAL4[Appl.G1a]. (Wentzell et al., 2012) SNF4Aγ[loe] mutant third instar larval brains show increased vacuolisation compared to controls. The phenotype is more severe in males than in females. An increased number of vacuoles is also seen in 4 day old adult flies. (Wentzell et al., 2012) Expression of Appl[Scer\UAS.cCSa] under the control of Scer\GAL4[Appl.G1a] substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Appl[s.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Appl.G1a] substantially suppresses the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Appl[dAICD.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Appl.G1a] is unable to suppress the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Appl[sd.Scer\UAS] under the control of Scer\GAL4[Appl.G1a] significantly enhances the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of Bace[Scer\UAS.cCSa] under the control of Scer\GAL4[Appl.G1a] enhances the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Expression of kuz[Scer\UAS.cFa] under the control of Scer\GAL4[Appl.G1a] suppresses the increase in vacuolisation seen in the brains of SNF4Aγ[loe] mutant third instar larvae. Hemizygosity for Appl[d] enhances the vacuolisation seen in 4 day old SNF4Aγ[loe] mutant males. Expression of Appl[Scer\UAS.cCSa] under the control of Scer\GAL4[Appl.G1a] partially suppresses the vacuolisation seen in 4 day old Appl[d] SNF4Aγ[loe] double mutant males, returning the phenotype to the level seen in SNF4Aγ[loe] single mutants. Expression of Appl[s.Scer\UAS.T:Ivir\HA1] under the control of Scer\GAL4[Appl.G1a] fails to suppress the vacuolisation seen in 4 day old Appl[d] SNF4Aγ[loe] double mutant males. (Wentzell et al., 2012) The addition of Hmgcr^clb1 or Hmgcr^clb2 to SNF4Aγ^loe animals shows a small but significant reduction in the number of holes seen in the adult brain. The addition of Hmgcr^{Scer\UAS.cvDa} (driven by Scer\GAL4^Appl.G1a) enhances the adult brain degeneration phenotype seen in SNF4Aγ^loe animals. The addition of Appl^d enhances the adult brain degeneration phenotype seen in SNF4Aγ^loe animals. (Tschaepe et al., 2002) Mutants exhibit severe vacuolisation of the central nervous system. The vacuolar pathology is most prominent around the central complex and the central parts of the brain. Neurons appear to undergo necrotic cell death. Mutants also have ~40% reduced levels of cholesterol ester. (Tschaepe et al., 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}SNF4Aγ^loe (Tschaepe et al., 2002, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 5 )
Research paper	Cook et al., 2012, PLoS ONE 7(9): e44440 Increased RhoA Prenylation in the loechrig (loe) Mutant Leads to Progressive Neurodegeneration. [FBrf0219441] Wentzell et al., 2012, Neurobiol. Disease 46(1): 78--87 Amyloid precursor proteins are protective in Drosophila models of progressive neurodegeneration. [FBrf0217742] Tschaepe et al., 2002, EMBO J. 21(23): 6367--6376 The neurodegeneration mutant lochrig interferes with cholesterol homeostasis and APPL processing. [FBrf0155731] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}SNF4Aγloe Insertion

Dmel\P{lacW}SNF4Aγ^loe Insertion