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General Information
Symbol
Dmel\P{PTT-GA}NrgG00305
Species
D. melanogaster
Name
FlyBase ID
FBti0027855
Also Known As
Nrg-GFP, G00305, Nrg::GFP
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
fertile, viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
X ( 7F4 )
Sequence Location
X:8,549,418..8,549,418 [+]
Target / Docking site
Member of Large Scale Dataset(s)
Description

Additional lines created; use of a high-throughput embryo sorter allowed screening of very large numbers of animals for GFP-expressing protein trap insertions.

A set of transgenic insertion stocks derived by TE mobilization using the protein-trap constructs P{PTT-GA}, P{PTT-GB}, and P{PTT-GC}. The constructs carry a w+mC mini-white visible marker and an Avic\GFP vital fluorescent protein-trap marker. In each of the three constructs, the splice acceptor and splice donor consensus sequences are in a different reading frame relative to the Avic\GFP sequence. For a successful GFP-positive insertion into an intron of a protein-coding gene, translation results in a fusion of the GFP to both the amino- and carboxyl-terminal parts of the trapped protein.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:8,549,418..8,549,418 [+]
Orientation
Cytological location (computed by FlyBase)
7F4 (inferred by FlyBase from sequence location)
Cytological location (reported)
7F3--7F4 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location

Insertion reported at coordinate 133625 on AE003444.1.

Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
Size (Kb)

5.956

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (14)
References (26)