Dmel\P{RS5}nopo^5-SZ-3004 Insertion

General Information
Symbol	Dmel\P{RS5}nopo^5-SZ-3004	Species	D. melanogaster
Name		FlyBase ID	FBti0029869
Feature type	transposable_element_insertion_site
Description
Inserted element	P{RS5}	Expression data
Affected gene(s)	nopo	Viability / fertility
Causes allele(s)	nopo^5-SZ-3004	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2R ( 55B12 )	Sequence location	2R:14,068,168..14,068,168 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{RS5}.DrosDel A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{RS5}. The P{RS5} construct carries the w[+mW] mini-white marker and Scer\FRT recombination sites (FBrf0091061); it serves as one recombination endpoint in the DrosDel deletion-generation system. (Ryder et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:14,068,168..14,068,168 [-] (Ryder and Roote, 2005.11.4)
Orientation	- (Ryder and Roote, 2005.11.4)
Cytological location (computed by FlyBase)	55B12 ( inferred by FlyBase from sequence location )
Cytological location (reported)	55B12 (reported as inferred from sequence location) (Ryder and Roote, 2005.11.4) 55B12 (reported as inferred from sequence location) (Ryder, 2003.6.17)
Comments concerning location
Sequence Data
Flanking sequence	AJ547318 (Ryder and Roote, 2005.11.4, Ryder, 2003.6.17)
Inserted Element
Construct	P{RS5}
Location-dependent role	deletion generation component (Golic and Golic, 1996) carries FRT site(s) (Golic and Golic, 1996)
Size	5.975Kb
Associated alleles	w^{+mW.Scer\FRT.hs}
Molecular map
Affected Gene(s)
Insertion may affect gene	nopo (Merkle et al., 2009, FlyBase Curators, 2008)
Alleles and Phenotypes
Causes alleles	nopo^5-SZ-3004 (Merkle et al., 2009, FlyBase Curators, 2008)

Lethality References lethal \| embryonic stage \| non-rescuable maternal effect (Merkle et al., 2009) lethal \| embryonic stage \| non-rescuable maternal effect \| recessive (Merkle et al., 2009)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference At 25[o]C, 62% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, 65% develop to the initiation of gastrulation or beyond, and 12% hatch. At 18[o]C, 78% of embryos derived from homozygous females mated to wild-type males develop to cycle 10 or beyond, 86% develop to the initiation of gastrulation or beyond, and 76% hatch. At 25[o]C, 67% of embryos derived from nopo[Z1447]/Df(2R)Exel7153 females mated to wild-type males develop to cycle 10 or beyond, 12% develop to the initiation of gastrulation or beyond, but none of them hatch. (Merkle et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Kyoto	125828 w¹¹¹⁸; P{RS5}nopo^5-SZ-3004
Linkouts
Comments
	Location 2R:13695525-13695526 refined by FlyBase alignment of dbGSS accession AJ547318 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	5-SZ-3004 (Merkle et al., 2009) P{RS5}5-SZ-3004 (Ryder, 2003.6.17, FlyBase, 2005, Ryder and Roote, 2005.11.4, FlyBase, 1992-) P{RS5}nopo^5-SZ-3004
Secondary FlyBase IDs

References ( 9 )
Research paper	Merkle et al., 2009, Development 136(3): 449--459 no poles encodes a predicted E3 ubiquitin ligase required for early embryonic development of Drosophila. [FBrf0206570] Ryder et al., 2004, Genetics 167(2): 797--813 The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. [FBrf0179424] Golic and Golic, 1996, Genetics 144(4): 1693--1711 Engineering the Drosophila genome: chromosome rearrangements by design. [FBrf0091061]
Personal communication to FlyBase	Ryder and Roote, 2005.11.4, Update from DrosDel Database - Release 4 mappings - November 2005. Update from DrosDel Database - Release 4 mappings - November 2005. [FBrf0188738] Ryder, 2003.6.17, Drosdel deletions. Drosdel deletions. [FBrf0161091]
FlyBase analysis	FlyBase Curators, 2013, Members of DrosDel insertion collections: P{RS3}, P{RS5}. Members of DrosDel insertion collections: P{RS3}, P{RS5}. [FBrf0220803] FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{RS5}nopo5-SZ-3004 Insertion

Dmel\P{RS5}nopo^5-SZ-3004 Insertion