A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}Tep4EY04656 Insertion

General Information
Symbol Dmel\P{EPgy2}Tep4EY04656 Species D. melanogaster
Name FlyBase ID FBti0033293
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Tep4 Viability / fertility fertile, viable
Causes allele(s) Tep4EY04656 Stock availability 1 publicly available
LINE ID EY04656
Genomic Location
Chromosomal location 2L ( 37F1 ) Sequence location 2L:19,549,893..19,549,893 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0014603 FBti0014604 FBti0014602 FBti0108589 FBti0028009 FBti0028007 FBti0105840 FBti0010860 FBti0021444 FBti0074776 FBti0046463 FBti0044744 FBti0143253 FBti0024238 FBti0108912 FBti0007647 FBti0104530 FBti0048693 FBti0112957 FBti0105020 FBti0021789 FBti0037434 FBti0070642 FBti0107081 FBti0047359 FBti0067695 FBti0054292 FBti0069458 FBti0024087 FBti0033293 FBti0104650 FBti0113450
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:19,549,893..19,549,893 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
37F1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
37F1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CC060547
(Gene Disruption Project members, 2001-, FlyBase, 1992-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Tep4
(Gene Disruption Project members, 2001-, Bou Aoun et al., 2011)
hide Alleles and Phenotypes
Causes alleles
Tep4EY04656
(Gene Disruption Project members, 2001-, Bou Aoun et al., 2011)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Mutant flies are not more susceptible to infection with either E. faecalis, M. marinum, L. monocytogenes, S.aureus, E. cloacae or S. pyogenes (via septic injury) than wild-type controls.
(Bou Aoun et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY04656
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
15936
y1 w67c23; P{EPgy2}Tep4EY04656
hide Linkouts
hide Comments
Location 2L:19545822-19545823 confirmed by FlyBase alignment of dbGSS accession CC060547 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}EY04656
(FlyBase, 1992-)
P{EPgy2}Tep4EY04656
 
P{EPgy2}TepIVEY04656
(FlyBase, 1992-, FlyBase, 2005)
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bou Aoun et al., 2011, J. Innate Immun. 3(1): 52--64
Analysis of thioester-containing proteins during the innate immune response of Drosophila melanogaster. [FBrf0212631]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]