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General Information
D. melanogaster
FlyBase ID
Also Known As
NP21, fru-GAL4, NP21-GAL4, fruNP21-Gal4, P{GawB}NP21
Inserted Element

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
Genomic Location
Chromosomal Location
3R ( 91B2 )
Sequence Location
3R:18,489,223..18,489,223 [-]
Target / Docking site
Member of Large Scale Dataset(s)

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:18,489,223..18,489,223 [-]
Cytological location (computed by FlyBase)
91B2 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)


Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Tissue/Position (including subcellular localization)
Additional Information

Drives expression in fruitless expressing neurons, including in male-specific fruitless neurons, in adults. Drives expression in the larval and adult mushroom bodies, and in a subset of pupal and adult antennal lobe glomeruli.

Scer\GAL4NP0021 drives expression in around 6 uniglomerular antennal lobe projection neurons. Their cell bodies are positioned in the lateral part of the antennal lobe and they project to antennal lobe glomerulus DA1 only. Expression is also detected in one ventrally located multiglomerular projection neuron which innervates glomeruli DA4l, VA1d, VA1v, VL1, VL2a, VM4, VM6, VC5, VP1, VP2, VP3 and VP4.

Scer\GAL4NP0021 drives expression in a subset of neurons in the male pupal brain, including a cluster of neurons located dorsal and medially to the antennal lobe (mAL neurons). Expression is also observed in a subset of neurons in the ventral nerve cord. Almost all neurons that express Scer\GAL4NP0021 also express hb protein. All neurons labelled by Scer\GAL4NP0021 express fru protein.

Expression is observed in ~200 projection neurons in the γ lobe of the mushroom body.

Expressed in clusters of mushroom body neurons that project to the median and lateral bundles. Expression in the corpora pedunculata (mushroom body) includes all known substructures (but may not include all neurons).

Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Stocks (2)
External Crossreferences and Linkouts ( 1 )
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

Location 3R:14314944-14314945 refined by FlyBase alignment of dbGSS accession AG215261 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

Synonyms and Secondary IDs (22)
References (34)