FB2020_06, released Dec 22, 2020

Insertion: Dmel\P{GawB}Hel89BNP1326

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General Information
Symbol
Dmel\P{GawB}Hel89BNP1326
Species
D. melanogaster
Name
FlyBase ID
FBti0034389
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{GawB}
Uses

GAL4 reporter/driver

Affected gene(s)
Hel89B
Viability / fertility
Associated allele(s)
Hel89BNP1326, Scer\GAL4NP1326
Stock availability
1 publicly available
LINE ID
Genomic Location
Chromosomal Location
3R ( 89A11 )
Sequence Location
3R:15,973,677..15,973,677 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:15,973,677..15,973,677 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
89A11 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG216223
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Location-dependent role
Size (Kb)

11.279

Associated alleles
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
Hel89B
(Olson et al., 2019, FlyBase Curators, 2008-)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
bilateral local interneuron of the adult antennal lobe | subset
(Tanaka et al., 2012)
antennal lobe hub | restricted
(Tanaka et al., 2012)
third instar larval stage
larval central brain
(Olson et al., 2019)
embryonic/larval dorsal vessel
(Olson et al., 2019)
Additional Information
Statement
Reference

Scer\GAL4NP1326 drives expression in around 4.5 local interneurons with cell bodies in the ventral region of the antennal lobe. Projections are detected to the antennal lobe hub and to all antennal lobe glomeruli except VM6, VP1, VP2 and VP3.

(Tanaka et al., 2012)
 
Marker for
Reflects expression of
Hel89B
(Tanaka et al., 2012)
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tanaka et al., 2012)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 3R:11799398-11799399 refined by FlyBase alignment of dbGSS accession AG216223 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
1326
(Olson et al., 2019)
NP1326
(Tanaka et al., 2012)
P{GawB}Hel89BNP1326
P{GawB}NP1326
(FlyBase, 1992-)
P{GawB}NP1326-5-1
(FlyBase, 2005, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (6)