FB2025_01 , released February 20, 2025
Insertion: Dmel\P{GawB}wakeNP1350
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General Information
Symbol
Dmel\P{GawB}wakeNP1350
Species
D. melanogaster
Name
FlyBase ID
FBti0034412
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
wake
Viability / fertility
Associated allele(s)
Scer\GAL4NP1350, wakeNP1350
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3R ( 94C1 )
Sequence Location
3R:22,667,144..22,667,144 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:22,667,144..22,667,144 [-]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
94C1 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG216170
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
wake
(Chen et al., 2022)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
bilateral local interneuron of the adult antennal lobe | subset
(Tanaka et al., 2012)
Additional Information
Statement
Reference

ScerGAL4NP1350 drives expression in 2 local interneurons with cell bodies in the ventral region of the antennal lobe. Projections are detected to glomerulus VM6 only.

(Tanaka et al., 2012)
 
Marker for
Reflects expression of
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tanaka et al., 2012)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 3R:18492865-18492866 refined by FlyBase alignment of dbGSS accession AG216170 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
NP1350
(Tanaka et al., 2012)
P{GawB}NP1350
(FlyBase, 1992-)
P{GawB}NP1350-5-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}wakeNP1350
wakeNP1350-Gal4
(Chen et al., 2022)
Name Synonyms
Secondary FlyBase IDs
    References (5)