FB2025_01 , released February 20, 2025
Insertion: Dmel\P{GawB}RhoGAP18BNP1514
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General Information
Symbol
Dmel\P{GawB}RhoGAP18BNP1514
Species
D. melanogaster
Name
FlyBase ID
FBti0034451
Feature type
transposable_element_insertion_site
Also Known As
whir3-GAL4
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
RhoGAP18B, Scer\GAL4
Viability / fertility
Associated allele(s)
RhoGAP18BNP1514, Scer\GAL4NP1514
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
X ( 18A3 )
Sequence Location
X:19,146,688..19,146,688 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:19,146,688..19,146,688 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
18A3 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG218218
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult mushroom body
(Rothenfluh et al., 2006)
adult pars intercerebralis | restricted
(Rothenfluh et al., 2006)
adult central complex | restricted
(Rothenfluh et al., 2006)
adult antennal lobe projection neuron | subset
(Rothenfluh et al., 2006)
adult gnathal ganglion | restricted
(Rothenfluh et al., 2006)
Additional Information
Statement
Reference

ScerGAL4RhoGAP18B-NP1514 drives expression in the adult mushroom body, parts of the pars intercerebralis, central complex and gnathal ganglion as well as in a subset of antennal lobe projection neurons that arborize on glomeruli DA1 and DM3.

(Rothenfluh et al., 2006)
 
Marker for
Reflects expression of
RhoGAP18B
(Rothenfluh et al., 2006)
Reporter construct used in assay
Avic\GFPUAS.cUa
(Rothenfluh et al., 2006)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location X:18980624-18980625 refined by FlyBase alignment of dbGSS accession AG218218 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
1514
(Olson et al., 2019)
P{GawB}NP1514
(FlyBase, 1992-)
P{GawB}NP1514-5-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}RhoGAP18BNP1514
whir3
(Rothenfluh et al., 2006)
whir3-GAL4
(Rothenfluh et al., 2006)
whir3-Gal4
(Acevedo et al., 2015, Peru Y Colón de Portugal et al., 2012)
Name Synonyms
Secondary FlyBase IDs
    References (9)