FB2020_06, released Dec 22, 2020

Insertion: Dmel\P{GawB}stjNP1574

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General Information
Symbol
Dmel\P{GawB}stjNP1574
Species
D. melanogaster
Name
FlyBase ID
FBti0034499
Feature type
transposable_element_insertion_site
Also Known As
stj-GAL4
Description
Inserted Element
P{GawB}
Uses

GAL4 reporter/driver

Affected gene(s)
Scer\GAL4, stj
Viability / fertility
Associated allele(s)
Scer\GAL4NP1574, stjNP1574
Stock availability
2 publicly available
LINE ID
Genomic Location
Chromosomal Location
2R ( 50C5 )
Sequence Location
2R:13,805,934..13,805,934 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:13,805,934..13,805,934 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
50C5 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG218357
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Location-dependent role
Size (Kb)

11.279

Associated alleles
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval brain
(Ly et al., 2008)
ventral nerve cord | restricted

Comment: in subset of motor neurons and interneurons

(Ly et al., 2008)
larval central brain
(Olson et al., 2019)
embryonic/larval wrapping glial cell of the eye disc
(Olson et al., 2019)
adult stage
adult brain
(Ly et al., 2008)
adult ventral nervous system | restricted
(Ly et al., 2008)
adult optic lobe
(Ly et al., 2008)
embryonic/larval salivary gland
(Ly et al., 2008)
photoreceptor cell

Comment: in axons and terminals

(Ly et al., 2008)
Additional Information
Statement
Reference

stj expression (assayed via stj-GAL4-driven GFP) is observed in the brain and ventral nerve cord of larvae and adults. In the visual system it is observed in the axons and terminals of photoreceptor cells as well as in the optic lobe. Comparison to other neuronal markers indicates that it is expressed in a subset of motor and interneurons in the larvarl CNS.

(Ly et al., 2008)
 
Marker for
Reflects expression of
stj
(Ly et al., 2008)
Reporter construct used in assay
Avic\GFPUAS.cUa
(Ly et al., 2008)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 2R:9321840-9321841 refined by FlyBase alignment of dbGSS accession AG218357 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
1574
(Olson et al., 2019)
NP1574-GAL4
(Ly et al., 2008)
P{GawB}NP1574
(FlyBase, 1992-)
P{GawB}NP1574-5-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}stjNP1574
stj-GAL4
(Ly et al., 2008)
stj-Gal4
(Neely et al., 2010)
Name Synonyms
Secondary FlyBase IDs
    References (7)