FB2025_01 , released February 20, 2025
Insertion: Dmel\P{GawB}amnNP2120
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General Information
Symbol
Dmel\P{GawB}amnNP2120
Species
D. melanogaster
Name
FlyBase ID
FBti0034629
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
amn, Scer\GAL4
Viability / fertility
Associated allele(s)
amnNP2120, Scer\GAL4NP2120
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
X ( 19A2 )
Sequence Location
X:19,887,128..19,887,128 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:19,887,128..19,887,128 [-]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
19A2 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG218774
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
amn
(FlyBase Curators, 2008-)
Scer\GAL4
(Pauls et al., 2010, Tanaka et al., 2008)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult dopaminergic PAM neuron
(Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2008)
mushroom body gamma lobe slice 4
(Tanaka et al., 2008)
mushroom body beta' anterior-posterior layer
(Tanaka et al., 2008)
mushroom body beta' lobe slice 1
(Tanaka et al., 2008)
mushroom body beta' lobe slice 2
(Tanaka et al., 2008)
mushroom body beta' middle layer
(Tanaka et al., 2008)
Additional Information
Statement
Reference

Projections of the mushroom body anterior inferior medial neurons innervate the anterior stratum of the beta' lobe segment 1, and/or throughout the middle and posterior strata of segment 2.

(Tanaka et al., 2008)
 
Marker for
adult antennal lobe projection neuron VL1 ilPN
(Tanaka et al., 2008)
Reflects expression of
amn
(Tanaka et al., 2008)
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tanaka et al., 2008)
Avic\GFPS65T.UAS
(Tanaka et al., 2008)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location X:19721064-19721065 refined by FlyBase alignment of dbGSS accession AG218774 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
NP2120
(Flybrain Neuron Database et al., 2018.6.28, Pauls et al., 2010, Tanaka et al., 2008)
P{GawB}NP2120
(FlyBase, 1992-)
P{GawB}NP2120-3-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}amnNP2120
Name Synonyms
Secondary FlyBase IDs
    References (7)