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General Information
Symbol
Dmel\P{GawB}sNPFNP6301
Species
D. melanogaster
Name
FlyBase ID
FBti0036921
Also Known As
snpf-Gal4, NP6301
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2L ( 38B1 )
Sequence Location
2L:20,027,886..20,027,886 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:20,027,886..20,027,886 [-]
Orientation
Cytological location (computed by FlyBase)
38B1 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4NP6301 drives expression in the ventral layers of the fan-shaped body, which the authors identify as layers 6-7, respectively.

Scer\GAL4NP6301 drives expression in distinct glomeruli in the adult antennal lobe. Except for expression in glomeruli DM2, VA2 and V, labelling is the same as the one seen with sNPF protein.

Scer\GAL4NP6301 labels two s-LNv Pdf neurons. These two s-LNv Pdf neurons are also labeled by expression of sNPF protein. Expression of Scer\GAL4NP6301 was also observed in a small set of Pdf-negative neurons with cell bodies in the eye disc and axons terminating in the larval optic neuropil.

Expression of Scer\GAL4NP6301 is similar in distribution to that seen with sNPF-XP. Scer\GAL4NP6301 appears to be expressed in most of the approximately 600 larval Kenyon cells, and in 190-200 cells of the larval central nervous system, 70 of which are in the brain (including the subesophageal ganglion) and a minimum of 120 in the thoracic and abdominal neuromeres. Three pairs of intensely labelled cells (referred to as LNCs) located dorsally in the protocerebrum are probably neurosecretory cells of the pars lateralis and have axons terminating in the ring gland and anterior aorta. Processes of Scer\GAL4NP6301 immunopositive larval cells are seen in neuropils of the protocerebrum, deutocerebrum, subesophageal ganglion, and most densely in the lobes of the mushroom body. Further processes are seen in the larval optic center, non-glomerular neuropils surrounding the mushroom body lobes, and the pars intercerebralis and lateralis. In the abdominal and thoracic neuromeres of the larval ventral nerve cord, Scer\GAL4NP6301 expressing cells are located dorsally, ventrally and laterally. In larval abdominal neuromeres A7 and A8 there are additional labelled cell bodies with large diameters, and a pair of large dorso-medially located cell bodies expressing Scer\GAL4NP6301 are found in abdominal segment A1. Two bundles of Scer\GAL4NP6301 labelled fibres are found in the ventral ganglia associated with the dors-median and ventro-median tracts. Expression of Scer\GAL4NP6301 in the adult brain generally colocalizes with sNPF protein, though the expression in photoreceptors R7 and R8 is ectopic. Expression of Scer\GAL4NP6301 in the lobula is seen in a set a of columnar neurons with cell bodies in the ventral base of the neuropil and a termination area in the lateral protocerebrum. Expression is strong in the distal lobula.

 
Marker for
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 2L:20023814-20023815 refined by FlyBase alignment of dbGSS accession AG218022 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

Synonyms and Secondary IDs (12)
References (12)