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General Information
Symbol
Dmel\P{GawB}NP6510
Species
D. melanogaster
Name
FlyBase ID
FBti0037001
Also Known As
NP6510, NP6510-GAL4
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
3L ( 73E5 )
Sequence Location
3L:17,049,509..17,049,509 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:17,049,509..17,049,509 [-]
Orientation
Cytological location (computed by FlyBase)
73E5 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4NP6510 drives expression in a subset of 15 dopaminergic PAM neurons that innervate beta- and beta'-lobes of the adult mushroom body. Projections are observed both to the ipsilateral and the contralateral side. Neurons innervating the beta'-lobe show extensive dendritic arborization. Two to three labelled neurons innervating the fan-shaped body are non-dopaminergic. GRASP experiments show that Scer\GAL4NP6510 expression is presynaptic in Kenyon cells of the mushroom body medial lobes.

The number of neurons detected by Scer\GAL4NP6510 varied between hemispheres of the same brain.

Scer\GAL4NP6510 drives expression in a subset of dopaminergic PAM neurons that innervate the mushroom body beta' lobe segment 2, beta lobe segment 1 and alpha lobe segment 1.

Approximately 7 fan-shaped F1 somata are labelled with Scer\GAL4NP6510. Neurites from these cells converge into a bundle that runs dorsally and the posteriorly into the fan-shaped body.

 
Marker for
Reflects expression of
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 3L:17014767-17014768 refined by FlyBase alignment of dbGSS accession AG219003 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

Synonyms and Secondary IDs (8)
References (20)