A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{5HPw+}CG33258A278 Insertion

General Information
Symbol Dmel\PBac{5HPw+}CG33258A278 Species D. melanogaster
Name FlyBase ID FBti0037659
Feature type transposable_element_insertion_site
Description
Inserted element PBac{5HPw+} Expression data
Affected gene(s) CG33258 Viability / fertility fertile, viable
Causes allele(s) CG33258A278 Stock availability 1 publicly available
LINE ID PA07987
Genomic Location
Chromosomal location 3L ( 72D1 ) Sequence location 3L:16,075,652..16,075,654 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0066252 FBti0152194 FBti0125728_2 FBti0125728_1 FBti0037659 FBti0045217 FBti0148396 FBti0127610 FBti0148219 FBti0027916 FBti0106496 FBti0027360 FBti0108014 FBti0112293 FBti0110687 FBti0029974 FBti0074701 FBti0112814 FBti0102238 FBti0125729_1 FBti0125729_2 FBti0018658 FBti0039668 FBti0109136 FBti0115530 FBti0111738 FBti0105193 FBti0103854
Member of Large Scale Dataset(s)
Dataset

PBac{5HPw[+]} is a 5' half P-element construct marked with the w[+mC] mini-white marker nested within a Tni\piggyBac-based construct; mobilization requires a source of Tni\piggyBac transposase.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{5HPw[+]}.
(Ring and Garza, 2003.6.10, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:16,075,652..16,075,654 [-]
(Berkeley Drosophila Genome Project, 2006.4.26)
Orientation
Cytological location
(computed by FlyBase)
72D1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
72D4 (reported as inferred from sequence location)
(Berkeley Drosophila Genome Project, 2006.4.26)
Comments concerning
location
hide Sequence Data
Flanking sequence
DX575156
(Berkeley Drosophila Genome Project, 2006.4.26)
DX575157
(Berkeley Drosophila Genome Project, 2006.4.26)
hide Inserted Element
Construct PBac{5HPw+}
Location-dependent
role
Size 6.938Kb
Associated alleles
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG33258
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
CG33258A278
(FlyBase Curators, 2008)
Lethality
References
viable
(Ring and Garza, 2003.6.10)
Sterility
References
fertile
(Ring and Garza, 2003.6.10)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
PA07987
(Berkeley Drosophila Genome Project, 2006.4.26)
Origin as a multiple insertion line
N
(Berkeley Drosophila Genome Project, 2006.4.26)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
16083
y1 w1118; PBac{5HPw+}CG33258A278
hide Linkouts
hide Comments
Resequencing confirmed original insertion site.
(Ring and Garza, 2003.6.10)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{5HPw+}A278
(FlyBase, 1992-)
PBac{5HPw+}CG33258A278
 
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Berkeley Drosophila Genome Project, 2006.4.26, BDGP TI submission.
BDGP TI submission. [FBrf0188739]
Ring and Garza, 2003.6.10, PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac.
PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. [FBrf0159884]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]
Thesis
Ring, 2001, Ph.D. Thesis, Florida State University, FL: xii + 219pp
Functional analysis of the Drosophila genome using transposable elements. [FBrf0152434]