Dmel\PBac{3HPy⁺}kune^C309 Insertion

General Information
Symbol	Dmel\PBac{3HPy⁺}kune^C309	Species	D. melanogaster
Name		FlyBase ID	FBti0037907
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{3HPy⁺}	Expression data
Affected gene(s)	kune	Viability / fertility
Causes allele(s)	kune^C309	Stock availability	1 publicly available
LINE ID	PC08916
Genomic Location
Chromosomal location	2R ( 41F6 )	Sequence location	2R:1,559,315..1,559,539 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:1,559,315..1,559,539 [+] (Berkeley Drosophila Genome Project, 2006.4.26)
Orientation
Cytological location (computed by FlyBase)	41F6 ( inferred by FlyBase from sequence location )
Cytological location (reported)	41F8 (reported as inferred from sequence location) (Berkeley Drosophila Genome Project, 2006.4.26)
Comments concerning location
Sequence Data
Flanking sequence	DX575588 (Berkeley Drosophila Genome Project, 2006.4.26) DX575589 (Berkeley Drosophila Genome Project, 2006.4.26) DX575890 (Berkeley Drosophila Genome Project, 2006.4.26)
Inserted Element
Construct	PBac{3HPy⁺}
Location-dependent role
Size	8.198Kb
Associated alleles	y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	kune (Berkeley Drosophila Genome Project, 2006.4.26, FlyBase Curators, 2008, Nelson et al., 2010)
Alleles and Phenotypes
Causes alleles	kune^C309 (FlyBase Curators, 2008, Nelson et al., 2010)

Lethality References lethal \| embryonic stage (Nelson et al., 2010) lethal \| embryonic stage \| recessive (Nelson et al., 2010)
Sterility References
Phenotype Manifest In
	embryonic/larval dorsal trunk (Nelson et al., 2010) embryonic/larval tracheal system (Nelson et al., 2010)
Detailed Description
Statement Reference At 23[o]C, expression of kune[Scer\UAS.cNa] under the control of Scer\GAL4[da.G32] only partially rescues the septate junction defects of kune[C309] mutants and does not rescue their embryonic lethality. At 28[o]C, expression of kune[Scer\UAS.cNa] under the control of Scer\GAL4[da.G32] rescues both the septate junction defects and embryonic lethality of kune[C309] mutants. (Nelson et al., 2010) A fluorescent 10kD dye injected into the body cavity readily leaks into the lumens of the trachea and salivary gland in mutant embryos, indicating a loss of paracellular barrier function. In addition, the fluorescent 10kD dye penetrates into the nerve cord of mutant embryos, indicating a defect in the blood-brain barrier. The length of the dorsal trunk of stage 16 homozygous and kune[C309]/Df(2R)BSC696 embryos is significantly increased compared to controls, and the mutant dorsal trunks appear tortuous. Mutant trachea have a diffuse, disorganized lumenal matrix (in contrast to wild-type trachea which contain a dense, fibrillar chitin cable) and lack the gap between the chitin cable and the apical surface of the cells that is seen in wild-type trachea. (Nelson et al., 2010) The tracheal length defects of kune[C309] pck[G0012] sinu[nwu7] triple mutant embryos are no more severe than those of single mutants. (Nelson et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	PC08916 (Berkeley Drosophila Genome Project, 2006.4.26)
Origin as a multiple insertion line	N (Berkeley Drosophila Genome Project, 2006.4.26)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	16333 y¹ w¹¹¹⁸; PBac{3HPy⁺}kune^C309/CyO
Linkouts
Comments
	Resequencing confirmed original insertion site. (Ring and Garza, 2003.6.10)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{3HPy}C309 (Nelson et al., 2010) PBac{3HPy⁺}C309 (FlyBase, 1992-) PBac{3HPy⁺}CG1298^C309 PBac{3HPy⁺}kune^C309
Secondary FlyBase IDs

References ( 8 )
Research paper	Nelson et al., 2010, Genetics 185(3): 831--839 The Drosophila Claudin Kune-kune Is Required for Septate Junction Organization and Tracheal Tube Size Control. [FBrf0211332] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Berkeley Drosophila Genome Project, 2006.4.26, BDGP TI submission. BDGP TI submission. [FBrf0188740] Berkeley Drosophila Genome Project, 2006.4.26, BDGP TI submission. BDGP TI submission. [FBrf0188739] Ring and Garza, 2003.6.10, PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. [FBrf0159884]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Thesis	Ring, 2001, Ph.D. Thesis, Florida State University, FL: xii + 219pp Functional analysis of the Drosophila genome using transposable elements. [FBrf0152434]

Dmel\PBac{3HPy+}kuneC309 Insertion

Dmel\PBac{3HPy⁺}kune^C309 Insertion