Dmel\P{GawB}MzVum Insertion

General Information
Symbol	Dmel\P{GawB}MzVum	Species	D. melanogaster
Name		FlyBase ID	FBti0037957
Feature type	transposable_element_insertion_site
Description
Inserted element	P{GawB}	Expression data	GAL4 reporter/driver
Affected gene(s)	Scer\GAL4, Vsx2	Viability / fertility
Causes allele(s)	Scer\GAL4^Vsx1-MzVum	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 5A3-5A5 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	5A3-5A5 ( near gene of known cytology )
Cytological location (reported)	5A5 (reported as inferred from sequence location) (Landgraf et al., 2003) 5A5 (in situ hybridization reported) (Landgraf et al., 2003)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{GawB}
Location-dependent role	GAL4 driver/enhancer trap (Brand and Perrimon, 1993)
Size	11.279Kb
Associated alleles	Scer\GAL4^wB w^+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene	Scer\GAL4 (Sanchez-Soriano and Prokop, 2005, Bottenberg et al., 2009, de Velasco et al., 2007, Landgraf et al., 2003, Sanchez-Soriano et al., 2005, Wheeler et al., 2006, Ting et al., 2011, Ting et al., 2011, Erclik et al., 2008, Erclik et al., 2008, Hadjieconomou et al., 2011) Vsx2 (Landgraf et al., 2003)
Alleles and Phenotypes
Causes alleles	Scer\GAL4^Vsx1-MzVum (Landgraf et al., 2003, Sanchez-Soriano and Prokop, 2005, Sanchez-Soriano et al., 2005, Bottenberg et al., 2009, Wheeler et al., 2006, de Velasco et al., 2007, Ting et al., 2011, Ting et al., 2011, Erclik et al., 2008, Erclik et al., 2008, Hadjieconomou et al., 2011)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference embryonic stage 12 -- third instar larval stage VUM neuron (Landgraf et al., 2003) embryonic stage 13 embryonic/larval pharynx (Erclik et al., 2008) pars intercerebralis (Erclik et al., 2008) larval outer optic anlage (Erclik et al., 2008) third instar larval stage ventral group vA neuron (Landgraf et al., 2003) embryonic/larval brain \| restricted (Erclik et al., 2008) outer optic anlage neuroblast (Erclik et al., 2008) lamina (Erclik et al., 2008) medulla (Erclik et al., 2008) larval outer optic anlage (Erclik et al., 2008) adult stage medulla (Erclik et al., 2008) optic lobe neuron \| subset (Ting et al., 2011) transmedullary neuron Tm5b (Ting et al., 2011)
Additional Information
Statement Reference The reporter is observed throughout the third instar larval outer optic lobe, including cells of the outer optic anlage, outer neuroblasts, lamina and medulla but not in the lobula complex or eye disc. (Erclik et al., 2008) The reporter is observed in the embryonic pharynx, pars intercerebralis, outer optic anlage, and in the adult medulla. (Erclik et al., 2008) Expression is observed in VUM motorneurons from embryonic stage 12 though third instar, in See diagram in Fig 4C of FBrf0160715. (Landgraf et al., 2003)

Marker for	VUM neuron (Landgraf et al., 2003)
Reflects expression of
Reporter construct used in assay	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} (Ting et al., 2011)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	29031 w^* P{GawB}MzVum; Syn⁹⁷
Linkouts
Comments
	Insertion 886bp upstream of Vsx2. The insertion reflects the expression of the Vsx1 gene located approximately 33kb from the insertion site. (Erclik et al., 2008) Insertion site is 886 base pairs upstream of Vsx2. (Landgraf et al., 2003)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	MzVUM-Gal4 (Landgraf et al., 2003, Sanchez-Soriano et al., 2005, Wheeler et al., 2006, Erclik et al., 2008, Erclik et al., 2008, Ting et al., 2011, Bottenberg et al., 2009, Ting et al., 2011) MzVum-Gal4 (Landgraf et al., 2003, Sanchez-Soriano and Prokop, 2005, de Velasco et al., 2007, Hadjieconomou et al., 2011) MzVum-Gal4MzVUM-Gal4 (Landgraf et al., 2003) P{GawB}MzVum (Landgraf et al., 2003, FlyBase, 1992-, ) VUM-Gal4 (Bottenberg et al., 2009)
Secondary FlyBase IDs

References ( 13 )
Research paper	Hadjieconomou et al., 2011, Nat. Methods 8(3): 260--266 Flybow: genetic multicolor cell labeling for neural circuit analysis in Drosophila melanogaster. [FBrf0213120] Ting et al., 2011, Genetics 188(1): 229--233 Focusing Transgene Expression in Drosophila by Coupling Gal4 With a Novel Split-LexA Expression System. [FBrf0213643] Bottenberg et al., 2009, Mech. Dev. 126(7): 489--502 Context-specific requirements of functional domains of the Spectraplakin Short stop in vivo. [FBrf0209450] Erclik et al., 2008, Curr. Biol. 18(17): 1278--1287 Conserved role of the Vsx genes supports a monophyletic origin for bilaterian visual systems. [FBrf0207154] de Velasco et al., 2007, Dev. Biol. 302(1): 309--323 Specification and development of the pars intercerebralis and pars lateralis, neuroendocrine command centers in the Drosophila brain. [FBrf0193772] Wheeler et al., 2006, Dev. Biol. 294(2): 509--524 Single-cell mapping of neural and glial gene expression in the developing Drosophila CNS midline cells. [FBrf0195284] Sanchez-Soriano et al., 2005, Dev. Biol. 288(1): 126--138 Are dendrites in Drosophila homologous to vertebrate dendrites? [FBrf0190157] Sanchez-Soriano and Prokop, 2005, J. Neurosci. 25(1): 78--87 The influence of pioneer neurons on a growing motor nerve in Drosophila requires the neural cell adhesion molecule homolog FasciclinII. [FBrf0184096] Landgraf et al., 2003, Dev. Biol. 260(1): 207--225 Charting the Drosophila neuropile: a strategy for the standardised characterisation of genetically amenable neurites. [FBrf0160715] Brand and Perrimon, 1993, Development 118(2): 401--415 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. [FBrf0064375]
Supplementary material	Ting et al., 2011, Genetics 188(1): Supporting Information. [FBrf0218391] Erclik et al., 2008, Curr. Biol. 18(17): Supplemental Data. [FBrf0207612]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]