A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{EPgy2}Cap-H2EY09979 Insertion

General Information
Symbol Dmel\P{EPgy2}Cap-H2EY09979 Species D. melanogaster
Name FlyBase ID FBti0038978
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Cap-H2, MED7 Viability / fertility fertile, viable
Causes allele(s) Cap-H2EY09979 Stock availability 1 publicly available
LINE ID EY09979
Genomic Location
Chromosomal location 3R ( 86C5 ) Sequence location 3R:6,586,731..6,586,731 [+]
Map ( GBrowse ) detailed view FBti0024674 FBti0100425 FBti0078314 FBti0037811 FBti0038978 FBti0102590 FBti0100822 FBti0036642 FBti0058302 FBti0048160 FBti0004944 FBti0126026_2 FBti0126026_1
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:6,586,731..6,586,731 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
86C5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
86C5 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CL526171
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Cap-H2
(Hartl et al., 2008)
MED7
(Hartl et al., 2008)
hide Alleles and Phenotypes
Causes alleles
Cap-H2EY09979
(Hartl et al., 2008)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
polytene chromosome & larval salivary gland
(Hartl et al., 2008, Hartl et al., 2008)
polytene chromosome & larval salivary gland | conditional - heat sensitive
(Hartl et al., 2008)
hide Detailed Description
Statement
Reference
Expression of Cap-H2[EY09979] under the control of Scer\GAL4[43B] results in polytene chromosome disassembly in the salivary gland nuclei of larvae. Expression of Cap-H2[EY09979] under the control of Scer\GAL4[hs.PB] in third instar larvae using a heat shock of 24 hours at 35[o]C results in polytene chromosome disassembly, with the extent of dispersal being directly related to the time after Cap-H2 induction that the salivary glands are examined. Significant disassembly of the polytene chromosomes is seen after 6 hours of Cap-H2 induction. Expression of Cap-H2[EY09979] under the control of Scer\GAL4[hs.PB] does not result in DNA breaks detectable with TUNEL in the salivary glands.
(Hartl et al., 2008)
Expression of Cap-H2[EY09979] under the control of Scer\GAL4[sd-SG29.1] partially suppresses the Ubx[1:Cbx-1]/+ wing phenotype.
(Hartl et al., 2008)
Expression of Cap-H2[EY09979] under the control of Scer\GAL4[hs.PB] results in a drastic separation of salivary gland chromosomal components.
(Hartl et al., 2008)
The polytene chromosome disassembly that is seen in larval salivary glands expressing Cap-H2[EY09979] under the control of Scer\GAL4[hs.PB] is suppressed by Cap-D3[EY00456]/Df(2L)Exel7023.
(Hartl et al., 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY09979
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17627
y1 w67c23; P{EPgy2}Cap-H2EY09979
hide Linkouts
hide Comments
Location 3R:6586731-6586732 confirmed by FlyBase alignment of dbGSS accession CL526171 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}Cap-H2EY09979
(FlyBase, 1992-)
P{EPgy2}EY09979
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Hartl et al., 2008, Science 322(5906): 1384--1387
Chromosome alignment and transvection are antagonized by condensin II. [FBrf0206308]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Supplementary material
Hartl et al., 2008, Science 322(5906):
Supporting Online Material. [FBrf0206401]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]