Dmel\P{SUPor-P}wisp^KG05287 Insertion

General Information
Symbol	Dmel\P{SUPor-P}wisp^KG05287	Species	D. melanogaster
Name		FlyBase ID	FBti0039041
Feature type	transposable_element_insertion_site
Description
Inserted element	P{SUPor-P}	Expression data
Affected gene(s)	wisp	Viability / fertility
Causes allele(s)	wisp^KG05287	Stock availability	1 publicly available
LINE ID	KG05287
Genomic Location
Chromosomal location	X ( 10F4 )	Sequence location	X:11,788,902..11,788,902 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{SUPor-P}.KG A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{SUPor-P}; created and vetted by the Gene Disruption Project (GDP). The P{SUPor-P} construct carries two visible markers: a w[+mC] mini-white marker flanked by su(Hw)-binding sites, designed to reduce the variablity of the phenotype of the white gene marker due position effects, in addition to the mini-yellow marker y[+mDint2]. (Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	X:11,788,902..11,788,902 [+] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	10F4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	10F4 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CL528237 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{SUPor-P}
Location-dependent role
Size	11.467Kb
Associated alleles	gypsy\su(Hw)BR⁴³⁰ w^BR.E.BR y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	wisp (Gene Disruption Project members, 2001-, Cui et al., 2008, Benoit et al., 2008)
Alleles and Phenotypes
Causes alleles	wisp^KG05287 (Gene Disruption Project members, 2001-, Cui et al., 2008, Benoit et al., 2008)

Lethality References lethal \| embryonic stage \| maternal effect (Benoit et al., 2008) lethal \| embryonic stage \| non-rescuable maternal effect \| recessive (Benoit et al., 2008) viable (Benoit et al., 2008)
Sterility References female sterile \| recessive (Benoit et al., 2008) male fertile (Benoit et al., 2008)
Phenotype Manifest In

Detailed Description
Statement Reference Oogenesis appears normal in homozygous females, and they lay the normal number of eggs, but embryos derived from homozygous or wisp[KG05287]/Df(1)RA47 females mated to wild-type males do not show any development. Embryos derived from wisp[KG05287]/Df(1)RA47 females do not complete meiosis. In 74% of embryos, a single meiotic spindle is visible which is thin or distorted and on which the chromosomes are scattered or separated in asymmetric pools. A second small spindle is sometimes present, which is nucleated by one or several lost chromosomes (8% of embryos). Among the remaining embryos, 15% are blocked in meiosis II; two meiotic spindles (which in most cases are abnormally arranged), can be seen. Meiotic figures in the mutant embryos correspond to either abnormal metaphase I or anaphase I. The karyosome in wisp[KG05287]/Df(1)RA47 stage 8 oocytes appears normal. 79% of stage 14 wisp[KG05287]/Df(1)RA47 oocytes do not maintain metaphase I arrest properly. The chromosomes separate along the spindle and do so asymmetrically, the fourth chromosome often migrating out of the spindle. The spindle is thin or irregular. (Benoit et al., 2008) The percentage of maternal effect embryonic lethality seen in embryos derived from orb[mel] homozygous females is increased by heterozygosity for wisp[KG05287]. The percentage of ventralised embryos is not increased by heterozygosity for one copy of wisp[KG05287]. (Benoit et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	KG05287 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	16467 y¹ P{SUPor-P}wisp^KG05287/FM7c
Linkouts
Comments
	Location X:11728806-11728807 confirmed by FlyBase alignment of dbGSS accession CL528237 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{SUPor-P}CG15737^KG05287 (FlyBase, 2005, Cui et al., 2008, FlyBase, 1992-) P{SUPor-P}KG05287 (FlyBase, 1992-) P{SUPor-P}wisp^KG05287 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 7 )
Research paper	Benoit et al., 2008, Development 135(11): 1969--1979 PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila. [FBrf0205291] Cui et al., 2008, Genetics 178(4): 2017--2029 Wispy, the Drosophila homolog of GLD-2, is required during oogenesis and egg activation. [FBrf0204618] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{SUPor-P}wispKG05287 Insertion

Dmel\P{SUPor-P}wisp^KG05287 Insertion