A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}EY10195 Insertion

General Information
Symbol Dmel\P{EPgy2}EY10195 Species D. melanogaster
Name FlyBase ID FBti0039637
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Viability / fertility fertile, viable
Causes allele(s) Stock availability 1 publicly available
LINE ID EY10195
Genomic Location
Chromosomal location 2R ( 52D12 ) Sequence location 2R:11,895,827..11,895,827 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0048757 FBti0106141 FBti0028173 FBti0071067 FBti0104578 FBti0003429 FBti0005192 FBti0109361 FBti0028283 FBti0011043 FBti0033783 FBti0025895 FBti0026199 FBti0110308 FBti0102671 FBti0109943 FBti0112943 FBti0072571 FBti0112366 FBti0102790 FBti0057143 FBti0099955 FBti0112958 FBti0039896 FBti0006589 FBti0024676 FBti0034069 FBti0115486 FBti0035866 FBti0056329 FBti0109021 FBti0021831 FBti0107616 FBti0108936 FBti0039637 FBti0042206 FBti0026100 FBti0049020 FBti0025689 FBti0036466 FBti0005283 FBti0039644 FBti0044058 FBti0100377 FBti0070974 FBti0076047 FBti0109506
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:11,895,827..11,895,827 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
52D12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
52D12 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CL528212
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hide Alleles and Phenotypes
Causes alleles
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY10195
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
16975
y1 w67c23; P{EPgy2}EY10195
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hide Comments
Location 2R:11523185-11523186 confirmed by FlyBase alignment of dbGSS accession CL528212 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}EY10195
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]