Dmel\P{EPgy2}opa1-like^EY09863 Insertion

General Information
Symbol	Dmel\P{EPgy2}opa1-like^EY09863	Species	D. melanogaster
Name		FlyBase ID	FBti0039803
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EPgy2}	Expression data
Affected gene(s)	opa1-like	Viability / fertility
Causes allele(s)	opa1-like^EY09863	Stock availability	2 publicly available
LINE ID	EY09863
Genomic Location
Chromosomal location	2R ( 50E4 )	Sequence location	2R:10,122,927..10,122,927 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EPgy2}.EY A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Bellen et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:10,122,927..10,122,927 [-] (Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)	50E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	50E4 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CL610258 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{EPgy2}
Location-dependent role	mobile activating element (UASG) (Bellen et al., 2004)
Size	10.908Kb
Associated alleles	Scer\UAS^14x.P\T TrlBR^cRa w^+mC y^+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene	opa1-like (Gene Disruption Project members, 2001-, Park et al., 2009, Yang et al., 2008)
Alleles and Phenotypes
Causes alleles	opa1-like^EY09863 (Gene Disruption Project members, 2001-, McQuibban et al., 2006, Park et al., 2009, Yang et al., 2008)

Lethality References lethal \| first instar larval stage (McQuibban et al., 2006)
Sterility References
Phenotype Manifest In
	indirect flight muscle (Park et al., 2010)
Detailed Description
Statement Reference Heterozygotes show defects in mitochondrial morphology in the indirect flight muscles, with smaller than normal mitochondria being seen between the muscle fibers. (Park et al., 2010) The downturned wing and crushed thorax phenotypes but not the flightless phenotype of Pink1[B9] mutant flies is significantly suppressed by opa1-like[EY09863]/+. The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1[B9] mutants is significantly rescued by opa1-like[EY09863]/+. (Park et al., 2009) No effect on mitochondrial network morphology in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster is seen in opa1-like[EY09863]/+ animals. (Yang et al., 2008) The abnormal wing posture phenotype and the abnormal morphology of the indirect flight muscles and their mitochondria which is seen in Pink1[B9] flies is suppressed by opa1-like[EY09863]/+. The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in Pink1[B9] animals is suppressed by opa1-like[EY09863]/+. The mitochondrial aggregation phenotype seen in the dorsolateral protocerebral posterior DA (dopaminergic) neuron cluster in animals expressing Pink1[Scer\UAS.cYa] under the control of Scer\GAL4[ple.PF] is not modified by opa1-like[EY09863]/+. (Yang et al., 2008) opa1-like^EY09863 mitotic clones in the male germline (i.e. mutant spermatids) exhibit irregularly-shaped nebenkerns, composed of many individual mitochondria that have coalesced beside the nucleus, but have failed to fuse. (McQuibban et al., 2006)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EY09863 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	20054 y¹ w^67c23; P{EPgy2}opa1-like^EY09863/CyO
Kyoto	114669 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}opa1-like^EY09863 /CyO y⁺
Linkouts
Comments
	Location 2R:9750285-9750286 confirmed by FlyBase alignment of dbGSS accession CL610258 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{EPgy2}CG8479 (Yang et al., 2008) P{EPgy2}CG8479^EY09863 (FlyBase, 2005, FlyBase, 1992-) P{EPgy2}EY09863 (FlyBase, 1992-) P{EPgy2}opa1-like^EY09863 (FlyBase, 1992-, ) unnamed (McQuibban et al., 2006)
Secondary FlyBase IDs

References ( 11 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Park et al., 2010, PLoS ONE 5(10): e13151 Drosophila Porin/VDAC Affects Mitochondrial Morphology. [FBrf0212060] Park et al., 2009, Biochem. Biophys. Res. Commun. 378(3): 518--523 The PINK1-Parkin pathway is involved in the regulation of mitochondrial remodeling process. [FBrf0207317] Yang et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(19): 7070--7075 Pink1 regulates mitochondrial dynamics through interaction with the fission/fusion machinery. [FBrf0205047] McQuibban et al., 2006, Curr. Biol. 16(10): 982--989 Normal mitochondrial dynamics requires rhomboid-7 and affects Drosophila lifespan and neuronal function. [FBrf0195303] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667] FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EPgy2}opa1-likeEY09863 Insertion

Dmel\P{EPgy2}opa1-like^EY09863 Insertion