A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}wmdEY06553 Insertion

General Information
Symbol Dmel\P{EPgy2}wmdEY06553 Species D. melanogaster
Name FlyBase ID FBti0039866
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) wmd Viability / fertility
Causes allele(s) wmdEY06553 Stock availability 2 publicly available
LINE ID EY06553
Genomic Location
Chromosomal location 2R ( 59E3 ) Sequence location 2R:19,435,493..19,435,493 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0057913 FBti0105708 FBti0057665 FBti0070872 FBti0059251 FBti0042971 FBti0126508 FBti0111176 FBti0026148 FBti0107723 FBti0029802 FBti0108316 FBti0030177 FBti0024655 FBti0100781 FBti0107731 FBti0039866 FBti0034659 FBti0036474 FBti0103888 FBti0024913 FBti0110846 FBti0051323 FBti0109033 FBti0112896 FBti0029071 FBti0070650 FBti0109964 FBti0110856 FBti0067088 FBti0106518 FBti0108216 FBti0005945 FBti0026277 FBti0100074 FBti0028938 FBti0005203 FBti0005211_1 FBti0005211_2 FBti0054124 FBti0068445 FBti0102171 FBti0007243 FBti0106506 FBti0108406 FBti0045822 FBti0125390_1 FBti0125390_2 FBti0112707 FBti0035305 FBti0071128 FBti0059326 FBti0126399 FBti0046451 FBti0042986 FBti0070790 FBti0025987
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:19,435,493..19,435,493 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
59E3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
59E3 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CL705806
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
wmd
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
wmdEY06553
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY06553
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
19854
y1 w67c23; P{EPgy2}wmdEY06553/CyO
Kyoto
114571
yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EPgy2}wmdEY06553 /CyO y+
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hide Comments
Location 2R:19055570-19055571 confirmed by FlyBase alignment of dbGSS accession CL705806 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}EY06553
(FlyBase, 2005, FlyBase, 1992-)
P{EPgy2}wmdEY06553
 
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]