A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}spinEY10097 Insertion

General Information
Symbol Dmel\P{EPgy2}spinEY10097 Species D. melanogaster
Name FlyBase ID FBti0039907
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) spin Viability / fertility fertile, viable
Causes allele(s) spinEY10097 Stock availability 1 publicly available
LINE ID EY10097
Genomic Location
Chromosomal location 2R ( 52E6 ) Sequence location 2R:12,012,503..12,012,503 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0125206_1 FBti0125206_2 FBti0047352 FBti0125207_2 FBti0125207_1 FBti0049557 FBti0039577 FBti0028905 FBti0102910 FBti0011072 FBti0039907 FBti0106566 FBti0007656 FBti0109722 FBti0125208_2 FBti0125208_1 FBti0030185 FBti0106447 FBti0026063 FBti0007704 FBti0107680 FBti0071159 FBti0047387 FBti0150235 FBti0150234 FBti0109567 FBti0103892 FBti0110833 FBti0113034 FBti0054101 FBti0059171 FBti0106146 FBti0034223 FBti0106020 FBti0108597 FBti0054994 FBti0104093 FBti0035442 FBti0036520 FBti0029885 FBti0106844 FBti0106802 FBti0070246 FBti0107466 FBti0007650 FBti0069065 FBti0035189 FBti0006553 FBti0034744
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:12,012,503..12,012,503 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
52E6 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
52E5 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CL705849
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
spin
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
spinEY10097
(Gene Disruption Project members, 2001-)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY10097
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
19895
y1 w67c23; P{EPgy2}spinEY10097
hide Linkouts
hide Comments
Location 2R:11639861-11639862 confirmed by FlyBase alignment of dbGSS accession CL705849 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}EY10097
(FlyBase, 1992-)
P{EPgy2}spinEY10097
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 6 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]