A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EPgy2}BuffyEY11259 Insertion

General Information
Symbol Dmel\P{EPgy2}BuffyEY11259 Species D. melanogaster
Name FlyBase ID FBti0040210
Feature type transposable_element_insertion_site
Description
Inserted element P{EPgy2} Expression data
Affected gene(s) Buffy Viability / fertility fertile, viable
Causes allele(s) BuffyEY11259 Stock availability 1 publicly available
LINE ID EY11259
Genomic Location
Chromosomal location 2R ( 48C1 ) Sequence location 2R:7,576,212..7,576,212 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0062541 FBti0071174 FBti0041956 FBti0064867 FBti0078457 FBti0040210 FBti0051996 FBti0026322 FBti0043060 FBti0010976 FBti0105179 FBti0007705 FBti0005218_2 FBti0111191 FBti0075246 FBti0071066 FBti0071131 FBti0005218_1 FBti0039808 FBti0105788 FBti0059480 FBti0072081 FBti0125113_1 FBti0049583 FBti0125113_2 FBti0067692 FBti0067615
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w[+mC] and the mini-yellow marker y[+mDint2], and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Bellen et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:7,576,212..7,576,212 [-]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location
(computed by FlyBase)
48C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
48C1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CL844429
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct P{EPgy2}
Location-dependent
role
mobile activating element (UASG)
(Bellen et al., 2004)
Size 10.908Kb
Associated alleles
Scer\UAS14x.P\T
TrlBRcRa
w+mC
y+mDint2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Buffy
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
BuffyEY11259
(Gene Disruption Project members, 2001-)
Lethality
References
viable
(Gene Disruption Project members, 2001-)
Sterility
References
fertile
(Gene Disruption Project members, 2001-)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EY11259
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
20280
y1 w67c23; P{EPgy2}BuffyEY11259
hide Linkouts
hide Comments
Location 2R:7204614-7204615 confirmed by FlyBase alignment of dbGSS accession CL844429 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EPgy2}BuffyEY11259
(FlyBase, 2005, FlyBase, 1992-, )
P{EPgy2}EY11259
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Sevrioukov et al., 2007, genesis 45(4): 184--193
Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development. [FBrf0202360]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}.
Members of GDP insertion collections: P{GT1}, P{SUPor-P}, P{EPgy2}, Mi{ET1}, Mi{MIC}. [FBrf0220667]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]