A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}Gr28bc01884 Insertion

General Information
Symbol Dmel\PBac{PB}Gr28bc01884 Species D. melanogaster
Name FlyBase ID FBti0040985
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) Gr28b Viability / fertility
Causes allele(s) Gr28bc01884 Stock availability 2 publicly available
LINE ID c01884
Genomic Location
Chromosomal location 2L ( 28A1 ) Sequence location 2L:7,459,215..7,459,215 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0106112 FBti0078333 FBti0040985 FBti0116087
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2L
(Thibault, 2004, Thibault, 2004)
Sequence Location
2L:7,459,215..7,459,215 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
28A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
27F6 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
28A1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ467529
(Gene Disruption Project members, 2001-)
CZ467530
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Gr28b
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Gr28bc01884
(Bloomington Drosophila Stock Center, 2004.11.6)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Gr28b[c01884] mutant flies eat at a significantly reduced rate compared to wild-type flies, and their ingestion volume is also reduced. Mutant flies also live longer than control flies. Gr28b[c01884] mutant flies infected with L. monocytogenes die faster than wild-type flies, with an average median time to death of 4 days compared to 6-7 days for infected wild-type flies. Gr28b[c01884] mutant flies infected with S. typhimurium live much longer than wild-type flies, with an average median time to death of 15 days compared to 8 days for infected wild-type flies. Gr28b[c01884] mutant flies infected with E. faecalis die at the same rate as infected wild-type flies. Less than 10% of Gr28b[c01884] mutants infected with either S. typhimurium or L. monocytogenes exhibit melanization, compared with over 90% of wild-type flies.
(Ayres and Schneider, 2009)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c01884
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10743
w1118; PBac{PB}Gr28bc01884
Harvard
-
PBac{PB}Gr28bc01884
hide Linkouts
hide Comments
Location 2L:7459214-7459215 confirmed by FlyBase alignment of dbGSS accessions CZ467530 and CZ467529 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c01884
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{PB}Gr28bc01884
(Thibault, 2004, FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Ayres and Schneider, 2009, PLoS Biol. 7(7): e1000150
The role of anorexia in resistance and tolerance to infections in Drosophila. [FBrf0208497]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]