A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}masc01899 Insertion

General Information
Symbol Dmel\PBac{PB}masc01899 Species D. melanogaster
Name FlyBase ID FBti0040987
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) mas Viability / fertility
Causes allele(s) masc01899 Stock availability 1 publicly available
LINE ID c01899
Genomic Location
Chromosomal location 3L ( 64A8 ) Sequence location 3L:4,166,100..4,166,100 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0025585 FBti0036040 FBti0035497 FBti0037078 FBti0072772 FBti0034821 FBti0024951 FBti0034380 FBti0104403 FBti0127657 FBti0149102 FBti0040987 FBti0140008 FBti0124445 FBti0105109 FBti0115913 FBti0145194
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3L
(Thibault, 2004, Thibault, 2004)
Sequence Location
3L:4,166,100..4,166,100 [-]
(FlyBase, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
64A8 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
64A9 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ467546
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
mas
(Bloomington Drosophila Stock Center, 2004.11.6)
hide Alleles and Phenotypes
Causes alleles
masc01899
(Bloomington Drosophila Stock Center, 2004.11.6)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c01899
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{PB}masc01899
hide Linkouts
hide Comments
Location 3L:4149631-4149632 determined by FlyBase alignment of dbGSS accession CZ467546 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c01899
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{PB}masc01899
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]