Dmel\PBac{PB}CHORD^c02881 Insertion

General Information
Symbol	Dmel\PBac{PB}CHORD^c02881	Species	D. melanogaster
Name		FlyBase ID	FBti0041072
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{PB}	Expression data
Affected gene(s)	CHORD	Viability / fertility
Causes allele(s)	CHORD^c02881	Stock availability	2 publicly available
LINE ID	c02881
Genomic Location
Chromosomal location	3R ( 95E8 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{PB}.Exel.c A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites. PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004, Thibault, 2004)
Sequence Location
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	95E8 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	PBac{PB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.257Kb
Associated alleles	w^+mPB.FRT
Molecular map
Affected Gene(s)
Insertion may affect gene	CHORD (FlyBase, 1992-)
Alleles and Phenotypes
Causes alleles	CHORD^c02881 (Bloomington Drosophila Stock Center, 2004.11.6)

Lethality References lethal \| third instar larval stage (Ferretti et al., 2010)
Sterility References
Phenotype Manifest In
	embryonic/larval salivary gland \| third instar larval stage (Ferretti et al., 2010) imaginal disc \| third instar larval stage (Ferretti et al., 2010) neuroblast \| third instar larval stage (Ferretti et al., 2010) testis \| third instar larval stage (Ferretti et al., 2010)
Detailed Description
Statement Reference Expression of CHORD[Scer\UAS.cFa] under the control of Scer\GAL4[Act5C.PI] in CHORD[c02881] mutant animals fails to rescue lethality. (Ferretti et al., 2010) CHORD[c02881]/+ flies are viable and morphologically normal. CHORD[c02881]/CHORD[c02881] flies die as third instar larvae. Mutant larvae are devoid of imaginal discs, and display strongly reduced testes and salivary glands. Brain preparations from CHORD[c02881] mutant larvae show that in 95% of dividing cells the chromosomes are elongated and irregularly condensed. Mutant cells spend more time in mitosis than wild-type control cells. The majority of cells remain arrested in a prophase-metaphase state. Of those that do escape into anaphase, strong defects in chromosome segregation are observed. 30% of mutant neuroblasts are polyploid, compared to 0.2% in control cells. In addition, an increase in the frequency of apoptotic cells is observed in mutant tissue (7.7%) compared to control brain tissue (0.5%). Supernumerary centrosomes are observed in CHORD[c02881] mutant brain cells. (Ferretti et al., 2010) Expression of Hsap\CHORDC1[Scer\UAS.cFa] under the control of Scer\GAL4[Act5C.PI] in CHORD[c02881] mutant flies rescues the imaginal disc and testis phenotype, and these tissues appear mostly normal. Most mitotic divisions observed in brain tissue are completely normal None of the observed mitotic figures were polyploid, and 98% of dividing cells display two centrosomes. These flies still die during metamorphosis. (Ferretti et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	c02881 (FlyBase, 1992-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11130 w¹¹¹⁸; PBac{PB}CHORD^c02881/TM6B, Tb¹
Harvard	- PBac{PB}CHORD^c02881
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{PB}c02881 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{PB}CHORD^c02881 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Ferretti et al., 2010, Dev. Cell 18(3): 486--495 Morgana/chp-1, a ROCK Inhibitor Involved in Centrosome Duplication and Tumorigenesis. [FBrf0210216] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{PB}CHORDc02881 Insertion

Dmel\PBac{PB}CHORD^c02881 Insertion