Dmel\PBac{PB}Pde1c^c04487 Insertion

General Information
Symbol	Dmel\PBac{PB}Pde1c^c04487	Species	D. melanogaster
Name		FlyBase ID	FBti0041148
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{PB}	Expression data
Affected gene(s)	Pde1c	Viability / fertility
Causes allele(s)	Pde1c^c04487	Stock availability	2 publicly available
LINE ID	c04487
Genomic Location
Chromosomal location	2L ( 33A2 )	Sequence location	2L:11,819,924..11,819,924 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004, Thibault, 2004)
Sequence Location	2L:11,819,924..11,819,924 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	33A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	33A2 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ469282 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{PB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.257Kb
Associated alleles	w^+mPB.FRT
Molecular map
Affected Gene(s)
Insertion may affect gene	Pde1c (Gene Disruption Project members, 2001-, Morton et al., 2010)
Alleles and Phenotypes
Causes alleles	Pde1c^c04487 (Bloomington Drosophila Stock Center, 2004.11.6, Morton et al., 2010)

Lethality References viable (Bloomington Drosophila Stock Center, 2004.11.6, Morton et al., 2010)
Sterility References fertile (Bloomington Drosophila Stock Center, 2004.11.6) male sterile (Morton et al., 2010) male sterile \| recessive (Morton et al., 2010)
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous males copulate significantly less frequently than their heterozygous siblings and than wild-type controls. Those males that do copulate take significantly longer to begin copulation than control males. Homozygous males spend a similar fraction of time engaged in courtship behaviour as control males. Some matings do result in sperm being successfully transferred to the female. 72 hours after mating, sperm are rarely detected in the reproductive system of females mated to homozygous males, suggesting that the sperm have either been eliminated or degraded. (Morton et al., 2010) Pde1c[c04487] males which are expressing Pde1c[RC.Scer\UAS] under the control of Scer\GAL4[Pde1c.RC] are partially fertile, with more than 50% of male-female pairs producing progeny. The number of progeny resulting from successful matings is similar to control matings. Pde1c[c04487] males which are expressing Pde1c[RC.Scer\UAS] under the control of Scer\GAL4[elav.PU] are partially fertile, with less than 25% of male-female pairs producing progeny. The copulation defects seen in Pde1c[c04487] males are completely rescued by expression of Pde1c[RC.Scer\UAS] under the control of either Scer\GAL4[Pde1c.RC] or Scer\GAL4[elav.PU]. (Morton et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	c04487 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	11440 w¹¹¹⁸; PBac{PB}Pde1c^c04487
Harvard	- PBac{PB}Pde1c^c04487
Linkouts
Comments
	Location 2L:11819924-11819925 confirmed by FlyBase alignment of dbGSS accession CZ469282 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{PB}c04487 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{PB}Pde1c^c04487 (FlyBase, 2005, FlyBase, 1992-, Morton et al., 2010)
Secondary FlyBase IDs

References ( 10 )
Research paper	Morton et al., 2010, Genetics 186(1): 159--165 Infertility and Male Mating Behavior Deficits Associated With Pde1c in Drosophila melanogaster. [FBrf0211842] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{PB}Pde1cc04487 Insertion

Dmel\PBac{PB}Pde1c^c04487 Insertion