A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}CG9850c05320 Insertion

General Information
Symbol Dmel\PBac{PB}CG9850c05320 Species D. melanogaster
Name FlyBase ID FBti0041215
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) CG9850 Viability / fertility
Causes allele(s) CG9850c05320 Stock availability 2 publicly available
LINE ID c05320
Genomic Location
Chromosomal location 2R ( 59F7 ) Sequence location 2R:19,641,259..19,641,259 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0051844 FBti0046667 FBti0051972 FBti0037170 FBti0055356 FBti0104546 FBti0072493 FBti0007732 FBti0039479 FBti0127531 FBti0041215 FBti0065836 FBti0043945 FBti0078332
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004, Thibault, 2004)
Sequence Location
2R:19,641,259..19,641,259 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
59F7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
59F7 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ469881
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG9850
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
CG9850c05320
(Bloomington Drosophila Stock Center, 2004.11.6)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c05320
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
17702
w1118; PBac{PB}CG9850c05320
Harvard
-
PBac{PB}CG9850c05320
hide Linkouts
hide Comments
Location 2R:19261336-19261337 confirmed by FlyBase alignment of dbGSS accession CZ469881 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c05320
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{PB}CG9850c05320
(FlyBase, 1992-, )
PBac{PB}Dimpc05320
(FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]