Dmel\PBac{RB}ranshi^e00091 Insertion

General Information
Symbol	Dmel\PBac{RB}ranshi^e00091	Species	D. melanogaster
Name		FlyBase ID	FBti0041356
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	ranshi	Viability / fertility
Causes allele(s)	ranshi^e00091	Stock availability	2 publicly available
LINE ID	e00091
Genomic Location
Chromosomal location	3R ( 85A9 )	Sequence location	3R:4,555,235..4,555,235 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004, Thibault, 2004)
Sequence Location	3R:4,555,235..4,555,235 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	85A9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	85A9 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ477996 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	ranshi (Gene Disruption Project members, 2001-, Lewandowski et al., 2010)
Alleles and Phenotypes
Causes alleles	ranshi^e00091 (Bloomington Drosophila Stock Center, 2004.11.6, Lewandowski et al., 2010)

Lethality References viable (Bloomington Drosophila Stock Center, 2004.11.6, Lewandowski et al., 2010)
Sterility References female sterile (Lewandowski et al., 2010) fertile (Bloomington Drosophila Stock Center, 2004.11.6) male fertile (Lewandowski et al., 2010)
Phenotype Manifest In
	germarium region 3 (Lewandowski et al., 2010) karyosome (Lewandowski et al., 2010)
Detailed Description
Statement Reference Homozygous males are viable, fertile and indistinguishable from wild type. Homozygous females fail to lay eggs. All egg chambers of homozygous females contain 16 germline cells, but they lack karyosomes. The nuclei of all the germline cells contain endoreplicated DNA that is typically seen in nurse cells. Spectrosome/fusome development appears indistinguishable from wild type in homozygous females. Meiosis is not restricted to a single cells in mutant germaria, as assessed by the presence of synaptonemal complexes in two adjacent cells within germarial region 3. In mutant cysts containing a single cell with synaptonemal complex, centrosomes accumulate within the cell containing the synaptonemal complex (as occurs in wild type), however, they are not detected within the posterior of the cell but are randomly placed within the cytoplasm. (Lewandowski et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e00091 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	17815 w¹¹¹⁸; PBac{RB}ranshi^e00091
Harvard	- PBac{RB}ranshi^e00091
Linkouts
Comments
	Location 3R:4555235-4555236 confirmed by FlyBase alignment of dbGSS accession CZ477996 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	pBac(RB)CG9793^e00091 (Lewandowski et al., 2010) PBac{RB}CG9793^e00091 (FlyBase, 2005, FlyBase, 1992-) PBac{RB}e00091 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{RB}ranshi^e00091
Secondary FlyBase IDs

References ( 10 )
Research paper	Lewandowski et al., 2010, Dev. Biol. 339(2): 307--319 Mago Nashi, Tsunagi/Y14, and Ranshi form a complex that influences oocyte differentiation in Drosophila melanogaster. [FBrf0210119] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{RB}ranshie00091 Insertion

Dmel\PBac{RB}ranshi^e00091 Insertion