A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}CG11073e00717 Insertion

General Information
Symbol Dmel\PBac{RB}CG11073e00717 Species D. melanogaster
Name FlyBase ID FBti0041425
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) CG11073 Viability / fertility
Causes allele(s) CG11073e00717 Stock availability 2 publicly available
LINE ID e00717
Genomic Location
Chromosomal location 2R ( 58B5-58B7 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
58B5-58B7 ( near gene of known cytology )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
carries FRT site(s)
Size 5.971Kb
Associated alleles
Molecular map
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Insertion may
affect gene
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Causes alleles
Lethality
References
Sterility
References
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Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
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Line ID
e00717
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
Harvard
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hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG11073e00717
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]