A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\PBac{RB}Mkk4e01485 Insertion

General Information
Symbol Dmel\PBac{RB}Mkk4e01485 Species D. melanogaster
Name FlyBase ID FBti0041504
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) Mkk4 Viability / fertility
Causes allele(s) Mkk4e01485 Stock availability 2 publicly available
LINE ID e01485
Genomic Location
Chromosomal location 3R ( 85A5 ) Sequence location 3R:4,473,306..4,476,824 [+]
Map ( GBrowse ) detailed view FBti0048644 FBti0037808 FBti0041504 FBti0126472 FBti0102682 FBti0014109 FBti0009967 FBti0068015 FBti0068017 FBti0125971_2 FBti0125971_1 FBti0106136 FBti0025189 FBti0103797 FBti0013445 FBti0028382 FBti0125972_2 FBti0125972_1 FBti0050344 FBti0125973_2 FBti0125973_1 FBti0043935 FBti0054527 FBti0009940 FBti0054560 FBti0112522 FBti0046299 FBti0143723 FBti0143860 FBti0065939 FBti0045453 FBti0143684 FBti0011774 FBti0111832 FBti0099881 FBti0109930 FBti0047607 FBti0106289 FBti0102220 FBti0109996 FBti0039957 FBti0108044 FBti0003112
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
3R
(Thibault, 2004, Thibault, 2004)
Sequence Location
3R:4,473,306..4,476,824 [+]
(Levis, 2009.8.28)
Orientation
+
(Thibault, 2004, Thibault, 2004, Levis, 2009.8.28)
Cytological location
(computed by FlyBase)
85A5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
Precise location cannot be determined from the available flanking sequence.
(Levis, 2009.8.28)
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Mkk4
(FlyBase, 1992-, Rallis et al., 2010)
hide Alleles and Phenotypes
Causes alleles
Mkk4e01485
(Rallis et al., 2010)
Lethality
References
Sterility
References
hide Phenotype Manifest In
Kenyon cell | somatic clone
(Rallis et al., 2010)
hide Detailed Description
Statement
Reference
Expression of Mkk4[Scer\UAS.T:Avic\GFP-YFP] using Scer\GAL4[tub] fully rescues the lethality associated with Mkk4[e01485]/Df(3R)p13. Expression of Mkk4[Scer\UAS.T:Avic\GFP-YFP] with Scer\GAL4[ey-OK107] rescues the axonal phenotypes seen in Mkk4[e01485] mushroom body clones.
(Rallis et al., 2010)
Mkk4[e01485] neuroblast clones in mushroom bodies show overextension of axons. Earlier born clones also show cell proliferation defects. Single cell Mkk4[e01485] clones in mushroom bodies show a low frequency of axon breaks.
(Rallis et al., 2010)
The axon overextension phenotype of Mkk4[e01485] neuroblast clones in mushroom bodies is suppressed by expression of hep[Scer\UAS.cBa] using Scer\GAL4[ey-OK107]. Single cell Mkk4[e01485], hep[r75] double mutant clones in mushroom bodies show a higher frequency of axon breaks than in clones of either single mutant.
(Rallis et al., 2010)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e01485
(FlyBase, 1992-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
17956
w1118; PBac{RB}Mkk4e01485/TM6B, Tb1
Harvard
-
PBac{RB}Mkk4e01485
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}e01485
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-, )
PBac{RB}Mkk4e01485
(FlyBase, 1992-, )
unnamed
(Rallis et al., 2010)
Secondary FlyBase IDs
hide References ( 7 )
Research paper
Rallis et al., 2010, Dev. Biol. 339(1): 65--77
Signal strength and signal duration define two distinct aspects of JNK-regulated axon stability. [FBrf0209975]
Thibault et al., 2004, Nature Genetics 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Levis, 2009.8.28, Location of e01485.
Location of e01485. [FBrf0208759]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]