Dmel\PBac{RB}mats^e03077 Insertion

General Information
Symbol	Dmel\PBac{RB}mats^e03077	Species	D. melanogaster
Name		FlyBase ID	FBti0041669
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	mats	Viability / fertility
Causes allele(s)	mats^e03077	Stock availability	2 publicly available
LINE ID	e03077
Genomic Location
Chromosomal location	3R ( 94A12 )	Sequence location	3R:18,247,539..18,247,595 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004, Thibault, 2004)
Sequence Location	3R:18,247,539..18,247,595 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	94A12 ( inferred by FlyBase from sequence location )
Cytological location (reported)	94A12 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ480841 (Gene Disruption Project members, 2001-) CZ480842 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	mats (Gene Disruption Project members, 2001-, He et al., 2005)
Alleles and Phenotypes
Causes alleles	mats^e03077 (Bloomington Drosophila Stock Center, 2004.11.6, He et al., 2005)

Lethality References lethal (He et al., 2005)
Sterility References
Phenotype Manifest In
	denticle (He et al., 2005) wing \| somatic clone (He et al., 2005) wing hair \| somatic clone (He et al., 2005) wing hair \| somatic clone \| supernumerary (He et al., 2005)
Detailed Description
Statement Reference Homozygous and hemizygous second instar larvae have many split denticles per segment. Homozygous clones in the wing can result in bulging of the wing surface. The cells in these clones appear to have a bulging apical surface and the wing hairs are located on an elevated pedestal. The wing hairs are often broader than normal. Homozygous clones in the wing can sometimes result in discrete outgrowths. Many cells in these outgrowths appear to have multiple wing hairs. Homozygous clones can result in abnormal pigmentation and outgrowths of clone tissue are seen. Homozygous clones in the pupal wing sometimes have a multiple wing hair phenotype. In many of the clones, hair initiation and outgrowth appear to be advanced compared to the neighbouring wild-type cells. If clones are induced early, the mutant cells are able to outcompete their wild-type neighbours and end up comprising most of the wing. (He et al., 2005) The multiple wing hair phenotype that is caused by expression of trc^{T453A.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 is dominantly enhanced by mats^e03077. fry¹ trc^S066917a mats^e03077 triple heterozygotes have a weak multiple wing hair phenotype in 40% of wings. fry² trc¹ mats^e03077 triple heterozygotes have a weak multiple wing hair phenotype in 41% of wings. The split denticle phenotype of mats^e03077 trc^S066917a double mutant second instar larvae is no more severe than the split denticle phenotype of each single mutant. (He et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e03077 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	18115 w¹¹¹⁸; PBac{RB}mats^e03077/TM6B, Tb¹
Harvard	- PBac{RB}mats^e03077
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB}CG13852^e03077 (Thibault, 2004, FlyBase, 1992-, He et al., 2005) PBac{RB}e03077 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{RB}mats^e03077 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	He et al., 2005, Molec. Biol. Cell 16(9): 4139--4152 Drosophila mob family proteins interact with the related tricornered (trc) and warts (wts) kinases. [FBrf0188034] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{RB}matse03077 Insertion

Dmel\PBac{RB}mats^e03077 Insertion