A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\PBac{RB}spz5e03444 Insertion

General Information
Symbol Dmel\PBac{RB}spz5e03444 Species D. melanogaster
Name FlyBase ID FBti0041710
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) spz5 Viability / fertility
Causes allele(s) spz5e03444 Stock availability 2 publicly available
LINE ID e03444
Genomic Location
Chromosomal location 3L ( 63A1 ) Sequence location 3L:2,887,990..2,887,990 [-]
Map ( GBrowse ) detailed view FBti0039009 FBti0050994 FBti0041710 FBti0055760 FBti0044478 FBti0065810 FBti0037920
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
3L
(Thibault, 2004, Thibault, 2004)
Sequence Location
3L:2,887,990..2,887,990 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
63A1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
63A1 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ481221
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
spz5
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
spz5e03444
(Bloomington Drosophila Stock Center, 2004.11.6)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6, Zhu et al., 2008)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6, Zhu et al., 2008)
hide Phenotype Manifest In
ISNb
(Zhu et al., 2008)
ISNd
(Zhu et al., 2008)
SNa
(Zhu et al., 2008)
hide Detailed Description
Statement
Reference
spz5[e03444]/Df(3L)Exel6092 transheterozygous mutant embryos exhibit an increase in apoptosis. Both ISNb/d and SNa projections are mildly affected in spz5[e03444]/Df(3L)Exel6092 mutant embryos.
(Zhu et al., 2008)
Loss of both NT1 and spz5 in spz5[e03444] NT1[41] double-mutant embryos results in an increase in apoptosis compared to each of the single mutants, revealing redundant or complementary functions in the control of neuronal survival. Viability is affected in NT1[41] spz5[e03444] double-mutants: in homozygosis, double mutant flies are viable (although some larval lethality, as well as when in trans over NT1[41] Df(3L)Exel6092, is observed), but NT1[41] spz5[e03444]/In(3LR)TM6B flies do not produce homozygous progeny at 18[o]C, suggesting unsuccessful larval competition. The penetrance of ISNb/d targeting defects increases in spz5[e03444] NT1[41] / Df(3L)Exel6092 NT1[41] double-mutant embryos, although not significantly. The penetrance of both SNa and ISNb/d targeting defects increases in spz5[e03444] NT1[41] spz[2], compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%). NT1[41] spz5[e03444] / NT1[41] Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.
(Zhu et al., 2008)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e03444
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
18155
w1118; PBac{RB}spz5e03444
Harvard
-
PBac{RB}spz5e03444
hide Linkouts
hide Comments
Location 3L:2871522-2871523 confirmed by FlyBase alignment of dbGSS accession CZ481221 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG9972e03444
(FlyBase, 1992-, FlyBase, 2005)
PBac{RB}e03444
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{RB}spz5e03444
 
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Zhu et al., 2008, PLoS Biol. 6(11): e284
Drosophila neurotrophins reveal a common mechanism for nervous system formation. [FBrf0206302]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nature Genetics 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Christensen et al., 2008.4.15, Isolation and characterization of Df(3L)BSC428.
Isolation and characterization of Df(3L)BSC428. [FBrf0204563]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.
Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]