A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\PBac{RB}Sin1e03756 Insertion

General Information
Symbol Dmel\PBac{RB}Sin1e03756 Species D. melanogaster
Name FlyBase ID FBti0041745
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) Sin1 Viability / fertility
Causes allele(s) Sin1e03756 Stock availability 2 publicly available
LINE ID e03756
Genomic Location
Chromosomal location 2R ( 51A2 ) Sequence location 2R:10,299,890..10,299,890 [-]
Map ( GBrowse ) detailed view FBti0049113 FBti0066197 FBti0041745 FBti0100873 FBti0035909 FBti0021565
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004, Thibault, 2004)
Sequence Location
2R:10,299,890..10,299,890 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
51A2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
51A2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ481507
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Sin1
(Gene Disruption Project members, 2001-, Hietakangas and Cohen, 2007, Koike-Kumagai et al., 2009)
hide Alleles and Phenotypes
Causes alleles
Sin1e03756
(Bloomington Drosophila Stock Center, 2004.11.6, Hietakangas and Cohen, 2007, Koike-Kumagai et al., 2009)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6, Hietakangas and Cohen, 2007)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6, Hietakangas and Cohen, 2007)
hide Phenotype Manifest In
abdominal anterior ventral multidendritic neuron vdaa
(Koike-Kumagai et al., 2009)
class IV dendritic arborizing neuron
(Koike-Kumagai et al., 2009)
class IV dendritic arborizing neuron | cell autonomous | somatic clone
(Koike-Kumagai et al., 2009)
dorsal multidendritic neuron ddaC
(Koike-Kumagai et al., 2009)
ventral multidendritic neuron vdaB
(Koike-Kumagai et al., 2009)
wing
(Hietakangas and Cohen, 2007)
hide Detailed Description
Statement
Reference
Class IV neuron-specific expression of Sin1[Scer\UAS.T:Zzzz\FLAG] largely rescues the dendritic phenotypes of single-cell Sin1[e03756] class IV dendrite arborization neuron clones. Expression of Sin1[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[ppk.PG] largely rescues the tiling defects seen in v\'ada and vdaB neurons of Sin1[e03756] mutants.
(Koike-Kumagai et al., 2009)
Homozygotes and Sin1[e03756]/Df(2R)BSC11 animals show a robust dendritic tiling defect in class IV dendrite arborization (da) neurons, with approximately 10% of dendritic branches crossing one another. In addition, the total number of dendrite branches is reduced to approximately 80% of wild type. Single-cell dorsal class IV da neuron clones that are homozygous for Sin1[e03756] (in a heterozygous background) show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. The v\'ada and vdaB dendrites often invade neighbouring dendritic fields in homozygous mutants, in contrast to what is seen in wild-type controls.
(Koike-Kumagai et al., 2009)
rictor[Δ2]/+ ; Sin1[e03756]/+ double heterozygotes have a dendritic tiling defect in class IV dendrite arborization (da) neurons, with a significantly higher number of dendritic branches crossing one another compared to wild type (neither single heterozygote shows this phenotype). rictor[Δ2] ; Sin1[e03756] double homozygotes show dendritic tiling defects in class IV da neurons that are indistinguishable from the single homozygotes. The dendritic branches of ddaC neurons in Tor[ΔP]/+ ; Sin1[e03756]/+ double heterozygous larvae show increased crossing over of each other, indicating a tiling defect. Class IV da neurons of Sin1[e03756]/+ ; trc[1]/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type. Expression of trc[Scer\UAS.cUa] under the control of Scer\GAL4[ppk.PG] slightly reduces the dendritic tiling defects seen in ddaC neurons in Sin1[e03756] animals. Expression of trc[Scer\UAS.T:Myr1] under the control of Scer\GAL4[ppk.PG] significantly rescues the dendritic tiling defects seen in ddaC neurons in Sin1[e03756] animals. Expression of trc[T449A.Scer\UAS.T:Myr1] under the control of Scer\GAL4[ppk.PG] does not rescue the dendritic tiling defects seen in ddaC neurons in Sin1[e03756] animals.
(Koike-Kumagai et al., 2009)
Sin1e03756 flies are normal in appearance and show a very modest delay of development. Sin1e03756/Df(2R)BSC11 flies show a modest reduction in tissue growth (wing area is reduced) compared to controls.
(Hietakangas and Cohen, 2007)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e03756
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
18188
w1118; PBac{RB}Sin1e03756
Harvard
-
PBac{RB}Sin1e03756
hide Linkouts
hide Comments
Location 2R:9927248-9927249 confirmed by FlyBase alignment of dbGSS accession CZ481507 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG10105e03756
(FlyBase, 1992-, FlyBase, 2005)
PBac{RB}e03756
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{RB}Sin1e03756
 
PBac e03756
(Koike-Kumagai et al., 2009)
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Koike-Kumagai et al., 2009, EMBO J. 28(24): 3879--3892
The target of rapamycin complex 2 controls dendritic tiling of Drosophila sensory neurons through the Tricornered kinase signalling pathway. [FBrf0209531]
Hietakangas and Cohen, 2007, Genes Dev. 21(6): 632--637
Re-evaluating AKT regulation: role of TOR complex 2 in tissue growth. [FBrf0201456]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nature Genetics 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Christensen et al., 2008.12.28, Isolation and characterization of Df(2R)BSC700.
Isolation and characterization of Df(2R)BSC700. [FBrf0206451]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.
Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]