A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}wole04276 Insertion

General Information
Symbol Dmel\PBac{RB}wole04276 Species D. melanogaster
Name FlyBase ID FBti0041803
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) wol Viability / fertility
Causes allele(s) wole04276 Stock availability 3 publicly available
LINE ID e04276
Genomic Location
Chromosomal location 2L ( 29A3 ) Sequence location 2L:8,302,610..8,302,610 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0043985 FBti0145177 FBti0046754 FBti0105110 FBti0056392 FBti0017599 FBti0045868 FBti0028228 FBti0034003 FBti0069362 FBti0110978 FBti0110885 FBti0041803 FBti0106393 FBti0076036 FBti0070923 FBti0027945 FBti0109975 FBti0041441 FBti0057944 FBti0042074 FBti0124654_2 FBti0046688 FBti0124654_1 FBti0024794
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2L
(Thibault, 2004, Thibault, 2004)
Sequence Location
2L:8,302,610..8,302,610 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
29A3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
29A3 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ482021
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
wol
(Gene Disruption Project members, 2001-, Haecker et al., 2008, Shaik et al., 2011)
hide Alleles and Phenotypes
Causes alleles
wole04276
(Bloomington Drosophila Stock Center, 2004.11.6, Haecker et al., 2008, Shaik et al., 2011)
Lethality
References
lethal | second instar larval stage
(Shaik et al., 2011)
Sterility
References
hide Phenotype Manifest In
cephalopharyngeal skeleton | germline clone
(Shaik et al., 2011)
denticle | germline clone
(Shaik et al., 2011)
embryonic/first instar larval cuticle | germline clone
(Shaik et al., 2011)
hide Detailed Description
Statement
Reference
Homozygous or wol[e04276]/Df(2L)BSC111 larvae hatch and have a wild type cuticle by light microscopy. They eventually die after one moult without displaying any obvious phenotype. Removal of maternal wol in wol[e04276] germline clones results in larvae with patterning and morphological defects, the most prominent being the failure to form a normal head skeleton. The cuticle is discontinuous and the body contours are irregular. At the edges of ruptures, the cuticle is often melanized. Specialized cuticle structures such as denticles are present. Removal of maternal and zygotic wol in wol[e04276] germline clones causes additional reduction in cuticle deposition and a complete failure of denticle formation and melanization.
(Shaik et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e04276
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 3 )
Bloomington
18243
w1118; PBac{RB}wole04276/CyO
Harvard
-
PBac{RB}wole04276
Kyoto
114515
yd2 w1118 P{ey-FLP.N}2; PBac{RB}wole04276 P{neoFRT}40A/CyO y+
hide Linkouts
hide Comments
Location 2L:8302610-8302611 confirmed by FlyBase alignment of dbGSS accession CZ482021 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}CG7870e04276
(FlyBase, 2005, FlyBase, 1992-)
PBac{RB}e04276
(Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
PBac{RB}wole04276
 
PBac RBe04276
(Haecker et al., 2008, Shaik et al., 2011)
Secondary FlyBase IDs
hide References ( 13 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Shaik et al., 2011, Glycobiology 21(6): 743--756
The Alg5 ortholog Wollknäuel is essential for correct epidermal differentiation during Drosophila late embryogenesis. [FBrf0214472]
Haecker et al., 2008, Development 135(10): 1745--1749
Wollknauel is required for embryo patterning and encodes the Drosophila ALG5 UDP-glucose:dolichyl-phosphate glucosyltransferase. [FBrf0204706]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]