Dmel\PBac{RB}wol^e04276 Insertion

General Information
Symbol	Dmel\PBac{RB}wol^e04276	Species	D. melanogaster
Name		FlyBase ID	FBti0041803
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	wol	Viability / fertility
Causes allele(s)	wol^e04276	Stock availability	3 publicly available
LINE ID	e04276
Genomic Location
Chromosomal location	2L ( 29A3 )	Sequence location	2L:8,302,610..8,302,610 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004, Thibault, 2004)
Sequence Location	2L:8,302,610..8,302,610 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	29A3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	29A3 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ482021 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	wol (Gene Disruption Project members, 2001-, Haecker et al., 2008, Shaik et al., 2011)
Alleles and Phenotypes
Causes alleles	wol^e04276 (Bloomington Drosophila Stock Center, 2004.11.6, Haecker et al., 2008, Shaik et al., 2011)

Lethality References lethal \| second instar larval stage (Shaik et al., 2011)
Sterility References
Phenotype Manifest In
	cephalopharyngeal skeleton \| germline clone (Shaik et al., 2011) denticle \| germline clone (Shaik et al., 2011) embryonic/first instar larval cuticle \| germline clone (Shaik et al., 2011)
Detailed Description
Statement Reference Homozygous or wol[e04276]/Df(2L)BSC111 larvae hatch and have a wild type cuticle by light microscopy. They eventually die after one moult without displaying any obvious phenotype. Removal of maternal wol in wol[e04276] germline clones results in larvae with patterning and morphological defects, the most prominent being the failure to form a normal head skeleton. The cuticle is discontinuous and the body contours are irregular. At the edges of ruptures, the cuticle is often melanized. Specialized cuticle structures such as denticles are present. Removal of maternal and zygotic wol in wol[e04276] germline clones causes additional reduction in cuticle deposition and a complete failure of denticle formation and melanization. (Shaik et al., 2011)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e04276 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	18243 w¹¹¹⁸; PBac{RB}wol^e04276/CyO
Harvard	- PBac{RB}wol^e04276
Kyoto	114515 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{RB}wol^e04276 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:8302610-8302611 confirmed by FlyBase alignment of dbGSS accession CZ482021 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB}CG7870^e04276 (FlyBase, 2005, FlyBase, 1992-) PBac{RB}e04276 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{RB}wol^e04276 PBac RB^e04276 (Haecker et al., 2008, Shaik et al., 2011)
Secondary FlyBase IDs

References ( 11 )
Research paper	Shaik et al., 2011, Glycobiology 21(6): 743--756 The Alg5 ortholog Wollknäuel is essential for correct epidermal differentiation during Drosophila late embryogenesis. [FBrf0214472] Haecker et al., 2008, Development 135(10): 1745--1749 Wollknauel is required for embryo patterning and encodes the Drosophila ALG5 UDP-glucose:dolichyl-phosphate glucosyltransferase. [FBrf0204706] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{RB}wole04276 Insertion

Dmel\PBac{RB}wol^e04276 Insertion