Dmel\PBac{RB}WRNexo^e04496 Insertion

General Information
Symbol	Dmel\PBac{RB}WRNexo^e04496	Species	D. melanogaster
Name		FlyBase ID	FBti0041827
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	WRNexo	Viability / fertility
Causes allele(s)	WRNexo^e04496	Stock availability	2 publicly available
LINE ID	e04496
Genomic Location
Chromosomal location	3R ( 91A3 )	Sequence location	3R:14,191,845..14,191,845 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004, Thibault, 2004)
Sequence Location	3R:14,191,845..14,191,845 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	91A3 ( inferred by FlyBase from sequence location )
Cytological location (reported)	91A3 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ482261 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	WRNexo (Gene Disruption Project members, 2001-, Saunders et al., 2008)
Alleles and Phenotypes
Causes alleles	WRNexo^e04496 (Bloomington Drosophila Stock Center, 2004.11.6, Saunders et al., 2008)

Lethality References viable (Bloomington Drosophila Stock Center, 2004.11.6)
Sterility References female sterile (Saunders et al., 2008) fertile (Bloomington Drosophila Stock Center, 2004.11.6) male fertile (Saunders et al., 2008)
Phenotype Manifest In

Detailed Description
Statement Reference Homozygotes have no gross morphological abnormality. Eggs derived from homozygous females do not hatch. Homozygous larvae are very sensitive to camptothecin (CPT) compared to controls. Surviving adults have roughened eyes and many die as pharate adults. Clones are recovered in the wing at a very high frequency in homozygous flies compared to control heterozygotes. In addition, some clones in the homozygous wing blades are very large, while the rate clones seen in heterozygous flies are all single cells. The majority of the clones recovered in the homozygous flies appear to be the result of mitotic DNA recombination events. (Saunders et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e04496 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	18267 w¹¹¹⁸; PBac{RB}WRNexo^e04496
Harvard	- PBac{RB}WRNexo^e04496
Linkouts
Comments
	Location 3R:14191845-14191846 confirmed by FlyBase alignment of dbGSS accession CZ482261 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB}CG7670^e04496 (FlyBase, 2005, FlyBase, 1992-) PBac{RB}e04496 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{RB}WRNexo^e04496 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 12 )
Research paper	Saunders et al., 2008, Aging Cell 7(3): 418--425 Identification and characterization of a Drosophila ortholog of WRN exonuclease that is required to maintain genome integrity. [FBrf0205216] Page et al., 2007, Fly 1(3): 172--181 A germline clone screen for meiotic mutants in Drosophila melanogaster. [FBrf0206085] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC509. Isolation and characterization of Df(3R)BSC509. [FBrf0204569] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{RB}WRNexoe04496 Insertion

Dmel\PBac{RB}WRNexo^e04496 Insertion