A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}Sema-2bf02042 Insertion

General Information
Symbol Dmel\PBac{WH}Sema-2bf02042 Species D. melanogaster
Name FlyBase ID FBti0042073
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) Sema-2b Viability / fertility
Causes allele(s) Sema-2bf02042 Stock availability 2 publicly available
LINE ID f02042
Genomic Location
Chromosomal location 2R ( 53C4 ) Sequence location 2R:12,312,794..12,312,794 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0042073 FBti0042740 FBti0101592
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004, Thibault, 2004)
Sequence Location
2R:12,312,794..12,312,794 [-]
(FlyBase, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
53C4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
53C4 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
53C4 (reported as inferred from sequence location)
(Parks et al., 2004)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ484538
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Sema-2b
(Sweeney et al., 2011, Bloomington Drosophila Stock Center, 2004.11.6)
hide Alleles and Phenotypes
Causes alleles
Sema-2bf02042
(Bloomington Drosophila Stock Center, 2004.11.6, Sweeney et al., 2011)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6, Sweeney et al., 2011)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
f02042
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
18505
w1118; PBac{WH}Sema-2bf02042
Harvard
-
PBac{WH}Sema-2bf02042
hide Linkouts
hide Comments
Location 2R:11940151-11940152 determined by FlyBase alignment of dbGSS accession CZ484538 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
f02042
(Sweeney et al., 2011)
PBac{WH}CG4383f02042
(FlyBase, 2005, FlyBase, 1992-)
PBac{WH}CG33960f02042
(FlyBase, 1992-)
PBac{WH}f02042
(Thibault, 2004, Thibault, 2004, Parks et al., 2004, FlyBase, 1992-)
PBac{WH}Sema-2bf02042
 
Secondary FlyBase IDs
hide References ( 13 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Sweeney et al., 2011, Neuron 72(5): 734--747
Secreted Semaphorins from Degenerating Larval ORN Axons Direct Adult Projection Neuron Dendrite Targeting. [FBrf0216932]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Parks et al., 2004, Nat. Genet. 36(3): 288--292
Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174230]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]