Dmel\PBac{WH}DCX-EMAP^f02655 Insertion

General Information
Symbol	Dmel\PBac{WH}DCX-EMAP^f02655	Species	D. melanogaster
Name		FlyBase ID	FBti0042147
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{WH}	Expression data
Affected gene(s)	DCX-EMAP	Viability / fertility
Causes allele(s)	DCX-EMAP^f02655	Stock availability	2 publicly available
LINE ID	f02655
Genomic Location
Chromosomal location	3L ( 71A2 )	Sequence location	3L:14,933,115..14,933,115 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3L (Thibault, 2004, Thibault, 2004)
Sequence Location	3L:14,933,115..14,933,115 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	71A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	71A2 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ485198 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{WH}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.234Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	DCX-EMAP (Gene Disruption Project members, 2001-)
Alleles and Phenotypes
Causes alleles	DCX-EMAP^f02655 (Bloomington Drosophila Stock Center, 2004.11.6)

Lethality References
Sterility References
Phenotype Manifest In
	Johnston organ (Bechstedt et al., 2010) sensillum campaniformium (Bechstedt et al., 2010)
Detailed Description
Statement Reference Flies homozygous for DCX-EMAP[f02655] or trans-heterozygous with either Df(3L)BSC441 or Df(3L)ED217 show mild uncoordination. Phenotypes include unstable walk, spread wing position, extensive grooming, inability to initiate stable flight, and sluggishness. The mechanically evoked compound action potentials that are recorded from the ears of control flies are absent in homozygous mutants. Homozygotes lack both auditory transducer gating and mechanical feedback amplification and thus fail to generate mechanically evoked electrical signals in the antennal nerve. Electron-dense materials in the tubular body of campaniform receptors of the haltere and the ciliary dilation of chordotonal receptors of Johnston\'s organ are absent in homozygotes. Despite these ultrastructural alterations, the general shape and size of the tubular body and the ciliary dilation are normal. (Bechstedt et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	f02655 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	18573 w¹¹¹⁸; PBac{WH}DCX-EMAP^f02655/TM6B, Tb¹
Harvard	- PBac{WH}DCX-EMAP^f02655
Linkouts
Comments
	Location 3L:14905273-14905274 refined by FlyBase alignment of dbGSS accession CZ485198 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{WH}CG13466^f02655 (FlyBase, 1992-, FlyBase, 2005) PBac{WH}CG42247^f02655 PBac{WH}DCX-EMAP^f02655 PBac{WH}f02655 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 11 )
Research paper	Bechstedt et al., 2010, Nat. Commun. 1: 11 A doublecortin containing microtubule-associated protein is implicated in mechanotransduction in Drosophila sensory cilia. [FBrf0212106] Bardin and Schweisguth, 2006, Dev. Cell 10(2): 245--255 Bearded family members inhibit Neuralized-mediated endocytosis and signaling activity of Delta in Drosophila. [FBrf0190191] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{WH}DCX-EMAPf02655 Insertion

Dmel\PBac{WH}DCX-EMAP^f02655 Insertion