A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{WH}Ttc19f05160 Insertion

General Information
Symbol Dmel\PBac{WH}Ttc19f05160 Species D. melanogaster
Name FlyBase ID FBti0042429
Feature type transposable_element_insertion_site
Description
Inserted element PBac{WH} Expression data
Affected gene(s) Ttc19 Viability / fertility
Causes allele(s) Ttc19f05160 Stock availability 2 publicly available
LINE ID f05160
Genomic Location
Chromosomal location 2L ( 37B11 ) Sequence location 2L:19,044,446..19,044,446 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0109276 FBti0025115 FBti0107286 FBti0145266 FBti0058857 FBti0047757 FBti0025978 FBti0037158 FBti0006795 FBti0007251 FBti0056516 FBti0042429 FBti0126642 FBti0051148 FBti0076070 FBti0026066 FBti0124858 FBti0111211 FBti0111200 FBti0076916 FBti0112317 FBti0072548 FBti0038991
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2L
(Thibault, 2004, Thibault, 2004)
Sequence Location
2L:19,044,446..19,044,446 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004, Thibault, 2004)
Cytological location
(computed by FlyBase)
37B11 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
37B11 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
37B11 (reported as inferred from sequence location)
(Parks et al., 2004)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ487785
(Gene Disruption Project members, 2001-)
CZ487786
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{WH}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.234Kb
Associated alleles
gypsy\su(Hw)BR348
Scer\UAS14x.hs.XP.Scer\FRT
Scer\UAS14x.hs.XP
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ttc19
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Ttc19f05160
(Bloomington Drosophila Stock Center, 2004.11.6)
Lethality
References
viable
(Bloomington Drosophila Stock Center, 2004.11.6)
Sterility
References
fertile
(Bloomington Drosophila Stock Center, 2004.11.6)
semi-sterile
(Ghezzi et al., 2011)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Mutant larvae appear normal, undergo normal pupation and develop into adults. Mutant flies have a reduced life span, with all flies dead by 35-40 days after eclosion compared to a life span of 80-90 days in controls, with fertility of approximately 60% that of controls. Spermatids look morphologically normal. Adult mutant flies show strong bang sensitivity, severely reduced spontaneous motor activity and impaired response in the optomotor test.
(Ghezzi et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
f05160
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
18843
w1118; PBac{WH}Ttc19f05160
Harvard
-
PBac{WH}Ttc19f05160
hide Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{WH}CG15173f05160
(FlyBase, 1992-, Thibault, 2004)
PBac{WH}f05160
(Thibault, 2004, Thibault, 2004, Parks et al., 2004, FlyBase, 1992-)
PBac{WH}Ttc19f05160
 
Secondary FlyBase IDs
hide References ( 13 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Ghezzi et al., 2011, Nat. Genet. 43(3): 259--263
Mutations in TTC19 cause mitochondrial complex III deficiency and neurological impairment in humans and flies. [FBrf0213121]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Parks et al., 2004, Nat. Genet. 36(3): 288--292
Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Parks et al., 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174230]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 2. [FBrf0174227]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]