Dmel\PBac{WH}cold^f05607 Insertion

General Information
Symbol	Dmel\PBac{WH}cold^f05607	Species	D. melanogaster
Name		FlyBase ID	FBti0042474
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{WH}	Expression data
Affected gene(s)	cold	Viability / fertility
Causes allele(s)	cold^f05607	Stock availability	3 publicly available
LINE ID	f05607
Genomic Location
Chromosomal location	2L ( 21E2 )	Sequence location	2L:574,741..574,741 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004, Thibault, 2004)
Sequence Location	2L:574,741..574,741 [-] (FlyBase, 2005)
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	21E2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	21D1 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ488208 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{WH}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.234Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	cold (Gene Disruption Project members, 2001-, Nilton et al., 2010, Hijazi et al., 2011, Syed et al., 2011)
Alleles and Phenotypes
Causes alleles	cold^f05607 (Bloomington Drosophila Stock Center, 2004.11.6, Gene Disruption Project members and Exelixis, 2005, Nilton et al., 2010, Hijazi et al., 2011, Syed et al., 2011)

Lethality References lethal (Nilton et al., 2010) lethal \| embryonic stage (Hijazi et al., 2011)
Sterility References
Phenotype Manifest In
	embryonic/larval dorsal trunk (Nilton et al., 2010) presumptive embryonic/larval tracheal system (Hijazi et al., 2011) subperineurial glial cell \| embryonic stage 17 (Syed et al., 2011)
Detailed Description
Statement Reference The overall organisation and branching pattern of the embryonic tracheal system is unaffected in cold[f05607] mutants. However, both the shape and the length of the tracheal dorsal trunk segments are abnormal in mutant flies. Differing from the wild-type, this structure appears in stage 15 embryos as a succession of bulging cysts and, by stage 16, as an abnormally convoluted tube. The fibrous structure of the tracheal chitin cable is disorganised in mutant embryos and chitin is deposited as an amorphous material in the tracheal lumen. In contrast to wild-type controls, soluble dye rapidly diffuses into the lumen of stage 16 cold[f05607] mutant trachea, indicating a role for cold in organisation of the paracellular barrier. cold[f05607] mutants do not exhibit defects in cell polarity or in the assembly of other adhesion structures. cold[f05607] embryos lacking the cold maternal contribution (but rescued paternally with a wild-type allele, using the FLP-DFS technique) do not show any phenotype and survive into adulthood. In contrast, mutants embryos lacking both maternal and zygotic contributions die during embryogenesis but do not display obvious morphological defects and are indistinguishable from embryos lacking only the cold zygotic contribution. A 10kDa dextran dye readily diffuses into the ventral cord of cold[f05607] mutant embryos, whereas it is efficiently excluded from this structure in wild-type embryos of the same age. Similarly, injected dye diffuses into the lumen of the chordotonal organs in stage 17 cold[f05607] embryos whereas septate junctions established between the cap, scolopal and ligament cells prevent dye intake in wild-type controls. (Hijazi et al., 2011) cold[f05607] mutants display a dye penetration phenotype where fluorescent dye diffuses across the blood-brain barrier into the CNS. The overall organization of the nervous system as well as the distribution of all glial cells, however, appears normal. Homozygous cold[f05607] mutant stage 17 embryos lack septae between subneurial glial cells, explaining the disrupted blood-brain barrier. (Syed et al., 2011) cold[f05607]/Df(2L)ED49 embryos develop elongated tracheal dorsal trunks and have disrupted tracheal paracellular barrier function (as assessed by dextran-dye injection). (Nilton et al., 2010)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	f05607 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 3 )
Bloomington	18890 w¹¹¹⁸; PBac{WH}cold^f05607/CyO
Harvard	- PBac{WH}cold^f05607
Kyoto	114662 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; PBac{WH}cold^f05607 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Exelixis, Inc. determined the insertion site of PBac{WH}Ipk2^f05607 to be at Release 3 genomic coordinate 576298 on arm 2L.The Gene Disruption project determined the insertion site of PBac{WH}Ipk2^f05607 to be at Release 3 genomic coordinate 576406 on arm 2L. (Deal and Cook, 2005.3.10) Location 2L:574741-574742 confirmed by FlyBase alignment of dbGSS accession CZ488208 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	coiled^P (Syed et al., 2011) P(WH)Bac^f05607 (Hijazi et al., 2011) PBac{WH}CG2813^f05607 (FlyBase, 2005, FlyBase, 1992-) PBac{WH}cold^f05607 PBac{WH}f05607 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{WH}Ipk2^f05607 (Deal and Cook, 2005.3.10, FlyBase, 1992-) PBac^f05607 (Syed et al., 2011)
Secondary FlyBase IDs

References ( 13 )
Research paper	Hijazi et al., 2011, PLoS ONE 6(3): e17763 The ly6 protein coiled is required for septate junction and blood brain barrier organisation in Drosophila. [FBrf0213269] Syed et al., 2011, J. Neurosci. 31(21): 7876--7885 The CD59 Family Member Leaky/Coiled Is Required for the Establishment of the Blood-Brain Barrier in Drosophila. [FBrf0213741] Nilton et al., 2010, Development 137(14): 2427--2437 Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components. [FBrf0211147] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Deal and Cook, 2005.3.10, Isolation and characterization of Df(2L)BSC107. Isolation and characterization of Df(2L)BSC107. [FBrf0182722] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{WH}coldf05607 Insertion

Dmel\PBac{WH}cold^f05607 Insertion