Dmel\PBac{WH}Smn^f05960 Insertion

General Information
Symbol	Dmel\PBac{WH}Smn^f05960	Species	D. melanogaster
Name		FlyBase ID	FBti0042509
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{WH}	Expression data
Affected gene(s)	Smn	Viability / fertility
Causes allele(s)	Smn^f05960	Stock availability	2 publicly available
LINE ID	f05960
Genomic Location
Chromosomal location	3L ( 73A9 )	Sequence location	3L:16,574,005..16,574,005 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3L (Thibault, 2004, Thibault, 2004)
Sequence Location	3L:16,574,005..16,574,005 [-] (Levis R., 2009.03.30)
Orientation	- (Thibault, 2004, Levis R., 2009.03.30, Thibault, 2004)
Cytological location (computed by FlyBase)	73A9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	73A9 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ488587 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{WH}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.234Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Smn (Chang et al., 2008, Shpargel et al., 2009, Rajendra et al., 2007)
Alleles and Phenotypes
Causes alleles	Smn^f05960 (Chang et al., 2008, Shpargel et al., 2009, Rajendra et al., 2007)

Lethality References lethal \| larval stage (Chang et al., 2008) lethal \| larval stage \| recessive (Chang et al., 2008, Rajendra et al., 2007) lethal \| recessive \| second instar larval stage (Shpargel et al., 2009) semi-viable (Chang et al., 2008)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Homozygous larvae are thinner and shorter than control larvae at 4 days after egg laying. Homozygous second-third instar larvae show impaired motor function, showing a smaller distance travelled in the 20 seconds after movement was stimulated with a needle than control larvae. (Shpargel et al., 2009) 3% of homozygotes survive as adult escapers. Smn[f05960]/Smn[f01109] third instar larvae do not show a change in bouton number per muscle area at the neuromuscular junction compared to wild type. (Chang et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	f05960 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	18923 w¹¹¹⁸; PBac{WH}Smn^f05960/TM6B, Tb¹
Harvard	- PBac{WH}Smn^f05960
Linkouts
Comments
	Error in previous genomic location reported in FlyBase (3L:16,557,625, r5): original sequence location data reported for release 3 genomic sequence; FlyBase error in promotion to subsequent releases. (Levis R., 2009.03.30)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	f05960 (Rajendra et al., 2007) f05960-5prime (Levis R., 2009.03.30) PBac{WH}f05960 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) PBac{WH}nxf2^f05960 (FlyBase, 1992-) PBac{WH}Smn^f05960 (FlyBase, 1992-, Shpargel et al., 2009)
Secondary FlyBase IDs

References ( 12 )
Research paper	Shpargel et al., 2009, Mol. Biol. Cell 20(1): 90--101 Gemin3 is an essential gene required for larval motor function and pupation in Drosophila. [FBrf0206864] Chang et al., 2008, PLoS ONE 3(9): e3209 Modeling spinal muscular atrophy in Drosophila. [FBrf0206033] Rajendra et al., 2007, J. Cell Biol. 176(6): 831--841 A Drosophila melanogaster model of spinal muscular atrophy reveals a function for SMN in striated muscle. [FBrf0194681] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Levis R., 2009.03.30, Additional mapped insertions (release 3 coordinates). Additional mapped insertions (release 3 coordinates). [FBrf0207436] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\PBac{WH}Smnf05960 Insertion

Dmel\PBac{WH}Smn^f05960 Insertion