Dmel\P{XP}escl^d01514 Insertion

General Information
Symbol	Dmel\P{XP}escl^d01514	Species	D. melanogaster
Name		FlyBase ID	FBti0042761
Feature type	transposable_element_insertion_site
Description
Inserted element	P{XP}	Expression data
Affected gene(s)	escl	Viability / fertility
Causes allele(s)	escl^d01514	Stock availability	2 publicly available
LINE ID	d01514
Genomic Location
Chromosomal location	2L ( 33B5 )	Sequence location	2L:11,990,983..11,990,983 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10	References Margueron et al., 2009, Nature 461(7265): 762--767
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004, Thibault, 2004)
Sequence Location	2L:11,990,983..11,990,983 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	33B5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	33B5 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ472282 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	P{XP}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.303Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	escl (Gene Disruption Project members, 2001-, Ohno et al., 2008)
Alleles and Phenotypes
Causes alleles	escl^d01514 (Bloomington Drosophila Stock Center, 2004.11.6, Gene Disruption Project members and Exelixis, 2005, Ohno et al., 2008)

Lethality References viable (Bloomington Drosophila Stock Center, 2004.11.6, Ohno et al., 2008)
Sterility References fertile (Bloomington Drosophila Stock Center, 2004.11.6, Ohno et al., 2008)
Phenotype Manifest In

Detailed Description
Statement Reference Flies zygotically mutant for both esc[6] and escl[d01514] produce larvae with poorly developed brain and imaginal discs, which die when they pupate. The lethality of esc[6], escl[d01514] double mutants is completely rescued by esc[T:Hsap\MYC]. The lethality of esc[6], escl[d01514] double mutants is not rescued by esc[F77A.T:Hsap\MYC], although the brain and imaginal disc phenotypes are alleviated by esc[F77A.T:Hsap\MYC]. The lethality of esc[6], escl[d01514] double mutants is not rescued by esc[F345A.T:Hsap\MYC]. (Margueron et al., 2009) Homozygous esc[6] escl[d01514] double mutants which have received maternal esc[+] function (as the female parents carry an esc[+] transgene on a balancer chromosome) survive through a prolonged third larval instar and die as early pupae. The double mutant larvae grow slowly. They have very small haltere and wing imaginal discs and the eye part of the eye-antenna disc is reduced in size, while the leg discs are nearly normal in size. The abdominal ganglia of the central nervous system are nearly normal in size in the double mutant larvae, but the optic lobes are approximately half normal diameter. Adult flies that are homozygous for escl[d01514] but heterozygous for esc[6] are viable, fertile and appear normal. Adult flies that are homozygous for esc[6] and heterozygous for escl[d01514] are few and very weak. They display a range of homeotic phenotypes, including transformations of antenna to leg, wing to haltere and A4 to A5, as well as having extra sex combs on the leg in males. (Ohno et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	d01514 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	19163 w¹¹¹⁸; P{XP}escl^d01514
Harvard	- P{XP}escl^d01514
Linkouts
Comments
	Location 2L:11990983-11990984 confirmed by FlyBase alignment of dbGSS accession CZ472282 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{XP}CG16969^d01514 (Bloomington Drosophila Stock Center, 2004.11.6, FlyBase, 1992-) P{XP}d01514 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-, Ohno et al., 2008) P{XP}escl^d01514 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Margueron et al., 2009, Nature 461(7265): 762--767 Role of the polycomb protein EED in the propagation of repressive histone marks. [FBrf0208990] Ohno et al., 2008, Mech. Dev. 125(5-6): 527--541 ESC, ESCL and their roles in Polycomb Group mechanisms. [FBrf0205395] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Cook and Cook, 2010.10.13, Isolation and characterization of Df(2L)BSC891. Isolation and characterization of Df(2L)BSC891. [FBrf0212103] Christensen and Cook, 2006.12.5, Isolation and characterization of Df(2L)BSC237. Isolation and characterization of Df(2L)BSC237. [FBrf0199226] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]

Dmel\P{XP}escld01514 Insertion

Dmel\P{XP}escl^d01514 Insertion