Dmel\P{XP}Slob^d07006 Insertion

General Information
Symbol	Dmel\P{XP}Slob^d07006	Species	D. melanogaster
Name		FlyBase ID	FBti0042870
Feature type	transposable_element_insertion_site
Description
Inserted element	P{XP}	Expression data
Affected gene(s)	Slob	Viability / fertility
Causes allele(s)	Slob^d07006	Stock availability	2 publicly available
LINE ID	d07006
Genomic Location
Chromosomal location	2L ( 28B4-28C1 )	Sequence location
Member of Large Scale Dataset(s)
Dataset	TI_set_P{XP}.Exel.d A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w[+mC] mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004, Thibault, 2004)
Sequence Location
Orientation	+ (Thibault, 2004, Thibault, 2004)
Cytological location (computed by FlyBase)	28B4-28C1 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{XP}
Location-dependent role	deletion generation component (Thibault et al., 2004) mobile activating element (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.303Kb
Associated alleles	gypsy\su(Hw)BR³⁴⁸ Scer\UAS^{14x.hs.XP.Scer\FRT} Scer\UAS^14x.hs.XP TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Slob (Bloomington Drosophila Stock Center, 2004.11.6, Munoz-Soriano et al., 2012)
Alleles and Phenotypes
Causes alleles	Slob^d07006 (Bloomington Drosophila Stock Center, 2004.11.6, Munoz-Soriano et al., 2012)

Lethality References viable (Bloomington Drosophila Stock Center, 2004.11.6)
Sterility References fertile (Bloomington Drosophila Stock Center, 2004.11.6)
Phenotype Manifest In

Detailed Description
Statement Reference Heterozygosity for Slob[d07006] does not enhance the wing phenotype caused by expression of two copies of Sep4[Scer\UAS.cMa] under the control of Scer\GAL4[da.G32]. (Munoz-Soriano et al., 2012)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	d07006 (FlyBase, 1992-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	19258 w¹¹¹⁸; P{XP}Slob^d07006
Harvard	- P{XP}Slob^d07006
Linkouts
Comments
	Exelixis, Inc. determined the insertion site of P{XP}Slob^d07006 to be at Release 3 genomic coordinate 7667292 on chromosome arm 2L, a site predicted to be within 28C1 on both the Release 3 and Release 4 genomic maps. (Christensen and Cook, 2006.8.29)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{XP}d07006 (Thibault, 2004, Thibault, 2004, FlyBase, 1992-) P{XP}Slob^d07006 (FlyBase, 1992-, Munoz-Soriano et al., 2012)
Secondary FlyBase IDs

References ( 9 )
Research paper	Munoz-Soriano et al., 2012, J. Mol. Neurosci. 48(1): 136--143 Septin 4, the Drosophila Ortholog of Human CDCrel-1, Accumulates in parkin Mutant Brains and is Functionally Related to the Nedd4 E3 Ubiquitin Ligase. [FBrf0219099] Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 2. [FBrf0174227] Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Christensen and Cook, 2006.8.29, Isolation and characterization of Df(2L)BSC191. Isolation and characterization of Df(2L)BSC191. [FBrf0199313] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{XP}Slobd07006 Insertion

Dmel\P{XP}Slob^d07006 Insertion