A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}walc00203 Insertion

General Information
Symbol Dmel\PBac{PB}walc00203 Species D. melanogaster
Name FlyBase ID FBti0043060
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) wal Viability / fertility
Causes allele(s) walc00203 Stock availability 1 publicly available
LINE ID c00203
Genomic Location
Chromosomal location 2R ( 48C1 ) Sequence location 2R:7,578,301..7,578,301 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0078457 FBti0040210 FBti0051996 FBti0026322 FBti0043060 FBti0010976 FBti0105179 FBti0007705 FBti0005218_2 FBti0111191 FBti0075246 FBti0071066 FBti0071131 FBti0005218_1 FBti0039808 FBti0105788 FBti0059480 FBti0072081 FBti0125113_1 FBti0049583 FBti0125113_2 FBti0067692 FBti0067615 FBti0114273 FBti0126654
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:7,578,301..7,578,301 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
48C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
48C2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ466269
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
wal
(FlyBase Curators, 2008)
hide Alleles and Phenotypes
Causes alleles
walc00203
(FlyBase Curators, 2008)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c00203
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{PB}walc00203
hide Linkouts
hide Comments
Location 2R:7206702-7206703 refined by FlyBase alignment of dbGSS accession CZ466269 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c00203
(Thibault, 2004, FlyBase, 2005, FlyBase, 1992-)
PBac{PB}walc00203
 
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]