A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}Sgt1c00608 Insertion

General Information
Symbol Dmel\PBac{PB}Sgt1c00608 Species D. melanogaster
Name FlyBase ID FBti0043266
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) Sgt1 Viability / fertility
Causes allele(s) Sgt1c00608 Stock availability 1 publicly available
LINE ID c00608
Genomic Location
Chromosomal location 3R ( 84F10 ) Sequence location 3R:4,130,156..4,130,156 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0070963 FBti0104235 FBti0066678 FBti0047282 FBti0104100 FBti0105156 FBti0065485 FBti0070912 FBti0042511 FBti0034387 FBti0027082 FBti0125965_1 FBti0125965_2 FBti0065806 FBti0045286 FBti0043266 FBti0042686 FBti0065074 FBti0065685 FBti0043543 FBti0043640 FBti0067594 FBti0131319 FBti0042914 FBti0050871 FBti0125966_2 FBti0125966_1 FBti0057889 FBti0058520
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3R
(Thibault, 2004)
Sequence Location
3R:4,130,156..4,130,156 [+]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
+
(Thibault, 2004)
Cytological location
(computed by FlyBase)
84F10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
84F10 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ466597
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Sgt1
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
Sgt1c00608
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c00608
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{PB}Sgt1c00608
hide Linkouts
hide Comments
Location 3R:4130155-4130156 refined by FlyBase alignment of dbGSS accession CZ466597 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c00608
(Thibault, 2004, FlyBase, 1992-)
PBac{PB}CG9617c00608
(FlyBase, 1992-, FlyBase, 2005)
PBac{PB}Sgt1c00608
 
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]