Dmel\PBac{PB}Sgt1^c01268 Insertion

General Information
Symbol	Dmel\PBac{PB}Sgt1^c01268	Species	D. melanogaster
Name		FlyBase ID	FBti0043543
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{PB}	Expression data
Affected gene(s)	Sgt1	Viability / fertility
Causes allele(s)	Sgt1^c01268	Stock availability	1 publicly available
LINE ID	c01268
Genomic Location
Chromosomal location	3R ( 84F10 )	Sequence location	3R:4,130,925..4,130,925 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{PB}.Exel.c A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites. PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3R (Thibault, 2004)
Sequence Location	3R:4,130,925..4,130,925 [-] (Gene Disruption Project members and Exelixis, 2005)
Orientation	- (Thibault, 2004)
Cytological location (computed by FlyBase)	84F10 ( inferred by FlyBase from sequence location )
Cytological location (reported)	84F10 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ467030 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{PB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	7.257Kb
Associated alleles	w^+mPB.FRT
Molecular map
Affected Gene(s)
Insertion may affect gene	Sgt1 (Gene Disruption Project members, 2001-, Martins et al., 2009)
Alleles and Phenotypes
Causes alleles	Sgt1^c01268 (Gene Disruption Project members and Exelixis, 2005, Martins et al., 2009)

Lethality References lethal (Martins et al., 2009) lethal \| recessive (Martins et al., 2009)
Sterility References
Phenotype Manifest In
	neuroblast (Martins et al., 2009)
Detailed Description
Statement Reference Third larval instar Sgt1[c01268] neuroblasts have abnormal mitotic phenotypes, including a high proportion of prometaphases with hypercondensed chromosomes and anaphases with lagging chromatids. Sgt1[c01268] has no overall effect upon mitotic index. There is a significant reduction in the frequency of prophases, a severe increase in the frequency of prometaphases and a reduction of cells exiting mitosis compared to control cells. Compared to controls, many fewer mutant neuroblasts incorporate BrdU. Treatment of Sgt1[c01268] neuroblasts with colchicine does not lead to the accumulation of cells in mitosis, in contrast to control cells. 22% of Sgt1[c01268] neuroblasts have monopolar spindles, with either a dispersed or well-focused centrosome at the centre. 30% of cells have just one centrosome and 20% have a diffuse centrosome. When Sgt1[c01268] larval neuroblasts are observed in primary culture, the following mitotic behaviours are observed; 55% enter mitosis with no apparent microtubule-organizing centre (MTOC), but after nuclear envelope breakdown (NEBD) are able to form a microtubule array that grows out from the kinetochores, they are unable to focus at the spindle poles and mitotic progression is delayed in prometaphase for more than 1 hours. 15% of the cells enter mitosis with a single MTOC, form a bipolar spindle and reach metaphase with a short delay but eventually exit mitosis with a significant delay (35 minutes after NEBD). 5% of the cells enter mitosis with more than two MTOCs, fail to organise a proper bipolar spindle and consequently fail to congress the chromosomes and arrest for long periods. 25% of the mutant neuroblasts enter mitosis with two MTOCs (as occurs in wild type). Only 40% of Sgt1[c01268] neuroblasts have a normal set of centriole pairs, approximately 40% have more than two centriole pairs and some cells have less than two centriole pairs. There is a clear correlation between increase in centriole number and increase in ploidy in the mutant cells. (Martins et al., 2009) Sgt1[c01268] ; Bub3[1] double mutant larval brain neuroblasts have a very low mitotic index. Compared to Sgt1[c01268] single mutant cells, the Sgt1[c01268] ; Bub3[1] double mutant cells enter mitosis more readily, do not accumulate in prometaphase and exit mitosis more frequently (as shown by the increase in anaphase and telophase figures). The double mutant cells do not show chromosome hypercondensation. Expression of polo[Scer\UAS.P\T.cMa] under the control of Scer\GAL4[da.G32] significantly rescues the mutant phenotypes of Sgt1[c01268] larval brain neuroblasts; there is an increase in cells with a normal number of centrosomes and a normal bipolar spindle and the cells show mostly normal mitotic progression. (Martins et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	c01268 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- PBac{PB}Sgt1^c01268
Linkouts
Comments
	Location 3R:4130925-4130926 confirmed by FlyBase alignment of dbGSS accession CZ467030 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	C01268 (Martins et al., 2009) PBac{PB}c01268 (Thibault, 2004, FlyBase, 1992-) PBac{PB}CG9617^c01268 (FlyBase, 2005, FlyBase, 1992-) PBac{PB}Sgt1^c01268 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Martins et al., 2009, EMBO J. 28(3): 234--247 Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization. [FBrf0207175] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{PB}Sgt1c01268 Insertion

Dmel\PBac{PB}Sgt1^c01268 Insertion