A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{PB}CG33054c02322 Insertion

General Information
Symbol Dmel\PBac{PB}CG33054c02322 Species D. melanogaster
Name FlyBase ID FBti0044060
Feature type transposable_element_insertion_site
Description
Inserted element PBac{PB} Expression data
Affected gene(s) CG33054, CG33056 Viability / fertility
Causes allele(s) CG33054c02322 Stock availability 1 publicly available
LINE ID c02322
Genomic Location
Chromosomal location 3L ( 78C2 ) Sequence location 3L:21,194,587..21,194,587 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063976 FBti0040839 FBti0058502 FBti0058503 FBti0050306 FBti0125837_2 FBti0125837_1 FBti0027466 FBti0046960 FBti0066634 FBti0143237 FBti0048091 FBti0070816 FBti0044060 FBti0055680 FBti0069256 FBti0024065 FBti0106587 FBti0106461 FBti0143642 FBti0108019 FBti0036186 FBti0057751 FBti0105328 FBti0034732
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{PB}. The PBac{PB} construct carries the w[+mC] mini-white marker flanked by short (48-bp) Scer\FRT recombination sites.
PBac{PB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
What does this section display?
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
3L
(Thibault, 2004)
Sequence Location
3L:21,194,587..21,194,587 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
78C2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
78C2 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ467861
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{PB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 7.257Kb
Associated alleles
w+mPB.FRT
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CG33054
(Gene Disruption Project members, 2001-)
CG33056
(Gene Disruption Project members, 2001-)
hide Alleles and Phenotypes
Causes alleles
CG33054c02322
(Gene Disruption Project members and Exelixis, 2005)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
c02322
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{PB}CG33054c02322
hide Linkouts
hide Comments
Location 3L:21135328-21135329 confirmed by FlyBase alignment of dbGSS accession CZ467861 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}c02322
(Thibault, 2004, FlyBase, 1992-)
PBac{PB}CG33054c02322
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]