Dmel\PBac{RB}RapGAP1^e00163 Insertion

General Information
Symbol	Dmel\PBac{RB}RapGAP1^e00163	Species	D. melanogaster
Name		FlyBase ID	FBti0046255
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	RapGAP1	Viability / fertility
Causes allele(s)	RapGAP1^e00163	Stock availability	1 publicly available
LINE ID	e00163
Genomic Location
Chromosomal location	2L ( 28B1 )	Sequence location	2L:7,574,288..7,574,378 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{RB}.Exel.e A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	2L (Thibault, 2004)
Sequence Location	2L:7,574,288..7,574,378 [+] (Gene Disruption Project members and Exelixis, 2005)
Orientation	+ (Thibault, 2004)
Cytological location (computed by FlyBase)	28B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	28B1 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ478060 (Gene Disruption Project members, 2001-) CZ478061 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	RapGAP1 (FlyBase Curators, 2008)
Alleles and Phenotypes
Causes alleles	RapGAP1^e00163 (FlyBase Curators, 2008)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e00163 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- PBac{RB}RapGAP1^e00163
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB}e00163 (Thibault, 2004, FlyBase, 1992-) PBac{RB}Rapgap1^e00163 PBac{RB}RapGAP1^e00163
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{RB}RapGAP1e00163 Insertion

Dmel\PBac{RB}RapGAP1^e00163 Insertion