Dmel\PBac{RB}Gyk^e00237 Insertion

General Information
Symbol	Dmel\PBac{RB}Gyk^e00237	Species	D. melanogaster
Name		FlyBase ID	FBti0046301
Feature type	transposable_element_insertion_site
Description
Inserted element	PBac{RB}	Expression data
Affected gene(s)	Gyk	Viability / fertility
Causes allele(s)	Gyk^e00237	Stock availability	1 publicly available
LINE ID	e00237
Genomic Location
Chromosomal location	3L ( 61B2 )	Sequence location	3L:203,669..203,669 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_PBac{RB}.Exel.e A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions. PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Thibault et al., 2004, Bellen et al., 2011)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	3L (Thibault, 2004)
Sequence Location	3L:203,669..203,669 [+] (FlyBase, 2005)
Orientation	+ (Thibault, 2004)
Cytological location (computed by FlyBase)	61B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	61B2 (reported as inferred from sequence location) (Gene Disruption Project members, 2001-)
Comments concerning location
Sequence Data
Flanking sequence	CZ478131 (Gene Disruption Project members, 2001-)
Inserted Element
Construct	PBac{RB}
Location-dependent role	deletion generation component (Thibault et al., 2004) carries FRT site(s) (Thibault et al., 2004)
Size	5.971Kb
Associated alleles	Rbp1^SA.RB w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Gyk (Gene Disruption Project members, 2001-, Wightman et al., 2012)
Alleles and Phenotypes
Causes alleles	Gyk^e00237 (Gene Disruption Project members and Exelixis, 2005, Wightman et al., 2012)

Lethality References
Sterility References fertile (Wightman et al., 2012)
Phenotype Manifest In

Detailed Description
Statement Reference Homozygotes are fertile, but fly cultures fail to thrive. When raised on a glycerol only food source, homozygous flies show reduced survival compared to control flies raised under the same conditions. (Wightman et al., 2012)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	e00237 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Harvard	- PBac{RB}Gyk^e00237
Linkouts
Comments
	Exelixis, Inc. determined the insertion site of PBac{RB}Gyk^e00237 to be at Release 3 genomic coordinate 184051 on arm 3L.The Gene Disruption Project determined the insertion site of PBac{RB}Gyk^e00237 to be at Release 3 genomic coordinate 184299 on arm 3L. (Andrade and Cook, 2005.8.25) Location 3L:187200-187201 refined by FlyBase alignment of dbGSS accession CZ478131 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	e00237 (Wightman et al., 2012) PBac{RB}e00237 (Thibault, 2004, FlyBase, 1992-) PBac{RB}Gyk^e00237 (FlyBase, 2005, Andrade and Cook, 2005.8.25, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 11 )
Research paper	Wightman et al., 2012, PLoS ONE 7(3): e31779 Glycerol hypersensitivity in a Drosophila model for glycerol kinase deficiency is affected by mutations in eye pigmentation genes. [FBrf0217823] Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Thibault et al., 2004, Nat. Genet. 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material	Thibault, 2004, Nature Genetics 36(3): Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase	Andrade and Cook, 2005.8.25, Isolation and characterization of Df(3L)BSC121. Isolation and characterization of Df(3L)BSC121. [FBrf0188668] Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file) Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177]
FlyBase analysis	FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\PBac{RB}Gyke00237 Insertion

Dmel\PBac{RB}Gyk^e00237 Insertion