A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\PBac{RB}thoc5e00906 Insertion

General Information
Symbol Dmel\PBac{RB}thoc5e00906 Species D. melanogaster
Name FlyBase ID FBti0046734
Feature type transposable_element_insertion_site
Description
Inserted element PBac{RB} Expression data
Affected gene(s) thoc5 Viability / fertility
Causes allele(s) thoc5e00906 Stock availability 1 publicly available
LINE ID e00906
Genomic Location
Chromosomal location 2R ( 60A13 ) Sequence location 2R:19,841,640..19,841,640 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0114857 FBti0125406_2 FBti0042100 FBti0125406_1 FBti0125407_1 FBti0125407_2 FBti0042390 FBti0043220 FBti0125408 FBti0046734 FBti0064794 FBti0033255
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w[+mC] mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Thibault et al., 2004, Bellen et al., 2011)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2R
(Thibault, 2004)
Sequence Location
2R:19,841,640..19,841,640 [-]
(Gene Disruption Project members and Exelixis, 2005)
Orientation
-
(Thibault, 2004)
Cytological location
(computed by FlyBase)
60A13 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
60A13 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Comments concerning
location
hide Sequence Data
Flanking sequence
CZ478739
(Gene Disruption Project members, 2001-)
hide Inserted Element
Construct PBac{RB}
Location-dependent
role
deletion generation component
(Thibault et al., 2004)
carries FRT site(s)
(Thibault et al., 2004)
Size 5.971Kb
Associated alleles
Rbp1SA.RB
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
thoc5
(Gene Disruption Project members, 2001-, Moon et al., 2011)
hide Alleles and Phenotypes
Causes alleles
thoc5e00906
(Gene Disruption Project members and Exelixis, 2005, Moon et al., 2011)
Lethality
References
Sterility
References
male sterile
(Moon et al., 2011)
male sterile | recessive
(Moon et al., 2011)
hide Phenotype Manifest In
spermatid
(Moon et al., 2011)
hide Detailed Description
Statement
Reference
thoc5[1]/thoc5[e00906] mutant testes contain primary spermatocytes but lack post-meiotic cells. thoc5[e00906] homozygotes and thoc5[1]/thoc5[e00906] trans-heterozygotes are male sterile.
(Moon et al., 2011)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
e00906
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Harvard
-
PBac{RB}thoc5e00906
hide Linkouts
hide Comments
Location 2R:19461717-19461718 confirmed by FlyBase alignment of dbGSS accession CZ478739 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{RB}e00906
(Thibault, 2004, FlyBase, 1992-)
PBac{RB}thoc5e00906
(FlyBase, 2005, FlyBase, 1992-, Moon et al., 2011, )
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Moon et al., 2011, Development 138(17): 3835--3845
The THO complex is required for nucleolar integrity in Drosophila spermatocytes. [FBrf0214600]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Supplementary material
Thibault, 2004, Nature Genetics 36(3):
Supplementary Table 3. [FBrf0174228]
Personal communication to FlyBase
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
FlyBase analysis
FlyBase Curators, 2013, Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}.
Members of Exelixis insertion collections: P{XP}, PBac{PB}, PBac{RB}, PBac{WH}. [FBrf0221061]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]